Antoine Rougier

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A monitoring programme was established in order to support community-based seaweed farming in south-west Madagascar by providing scientific information on the effects of physico-chemical and health factors influencing the growth of Kappaphycus alvarezii (cottonii). Six aquaculture site configurations were studied. These consisted of high and low flow(More)
The in vivo relationship between the reservoir effect of the stratum corneum and the percutaneous absorption was established in both human volunteers and hairless rats. Four doses of [ring-14C]benzoic acid, 125, 250, 500, and 1000 nmol, were applied on 1 cm2 either on the external side of the human arm or on the back of the rat, for 30 min. In both human(More)
A relationship between stratum corneum reservoir function and percutaneous absorption has been established in the hairless rat. Two hundred nanomoles of 10 substances that have a wide range of chemical structures were topically applied for 30 min and the total body distribution was measured after 96 h. The quantity of substance present in the stratum(More)
We tested the effect of various imidazole derivatives applied topically, on P-450-dependent enzyme activity of a reconstructed epidermis in conditions simulating clinical use. At nontoxic concentrations (determined by a cytotoxicity test based on the reduction of a tetrazolium salt, MTT, by mitochondrial deshydrogenase) econazole and clotrimazole had a(More)
The influence of nine vehicles on in vivo percutaneous absorption of benzoic acid has been studied in the hairless rat. Although the vehicles used were simple in composition, the total amount of benzoic acid which penetrated varied by a factor of 50. A weak relationship was found between penetration of benzoic acid and its maximum solubility in the(More)
Percutaneous absorption of 4 radiolabeled molecules was compared in the hairless rat (back) and in different anatomic sites in human (arm, abdomen, postauricular, forehead). The conditions under which these 4 compounds were administered (area treated, dose, vehicle, contact time, etc.) were similar in both species. The results showed that, in humans and(More)
The relation existing in vivo between the reservoir function of the stratum corneum following a 30-min application time and the total percutaneous absorption of molecules applied for a longer time was studied on hairless rats. One thousand nanomoles of 4 radiolabeled molecules (theophylline, nicotinic acid, acetyl salicylic acid, benzoic acid) were applied(More)
The Episkin epidermal system is a reconstituted organotypic culture of human keratinocytes forming a multilayer differentiated epidermis on a collagen matrix. This standardized model was used to measure various parameters reflecting irritation observed in vivo following topical application of a variety of surfactants. A comparison of the results with(More)
As they simulate the multilayer tissue architecture present in vivo, three-dimensional (3D) cultures of skin epithelial cells represent an attractive alternative for in vitro irritancy testing. In this study histology of non-commercial (Skin recombinant on de-epidermized dermis) and commercial models (Episkin, ZK 1300, Living Skin Equivalent) was compared.(More)
Exposure to UV radiation has been shown to induce cutaneous biological changes and chemical modifications of exogenous compounds. The application of sunscreen limits UV-induced cutaneous injury. Besides damages can also be elicited by some chemicals following UV irradiation (phototoxic agents). The effects of UVA and UVB irradiation have been studied in(More)