Anthony P. Pagano

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Gallbladder motility is impaired in chroniccholelithiasis but has not been studied in acuteacalculous cholecystitis. The aim of this study was todetermine the effects of acute acalculous inflammation on gallbladder contractility using the commonbile duct ligation (CBDL) model in guinea pigs. Threegroups of guinea pigs were studied: CBDL, normal, andsham(More)
The aim of this study was to determine the functional role of muscarinic receptor subtypes regulating gallbladder cholinergic contractions. Electrical field stimulation (EFS; 16 Hz) produced contractile responses of guinea pig gallbladder muscle strips in vitro that were inhibited by 1 microM tetrodotoxin (2 +/- 2% of control) and 1 microM atropine (1 +/-(More)
Gallbladder motility is modulated by intrinsic nerves, the identities of which are not well established. The aim of this study was to determine the effect of nicotinic receptor stimulation of intrinsic nerves on gallbladder muscle contractility. Guinea pig gallbladder muscle strips were studied in vitro. Histamine 1 microM was used to increase baseline(More)
UNLABELLED The aim of this study was to investigate the role of extracellular Ca2+ utilization in cholecystokinin (CCK) and acetylcholine-induced guinea pig gallbladder contractions by using agents that modulate influx of extracellular Ca2+ through voltage-dependent calcium channels. METHODS Guinea pig gallbladder muscle strips were studied isometrically(More)
Gallbladder motility is modulated by intrinsiccholinergic neurons. The aims of this study were todetermine: (1) the effect of electric field stimulation(EFS) on guinea pig gallbladder smooth muscle, and (2) the role of calcium channels inmediating neurotransmitter release. Gallbladder musclestrips were studied isometrically in vitro. EFS (1-16Hz, 100 V,(More)
Histamine type 2 receptor antagonists (H2RAs)have been found to alter gastric motility. The aims ofthis study were to determine if H2RAs affect antralcontractility in vitro and the mechanism of this effect. Guinea pig antral muscle strips werepinned in an organ bath after removing the mucosa, andcircular muscle tension was measured using an isometricforce(More)
The aim of this study was to characterize the receptor(s) for bombesin (BN) and its homologues (gastrin releasing peptide, GRP; neuromedin B, NMB; neuromedin C, NMC) in guinea pig gallbladder muscle strips. Dose-dependent contractions were induced by all peptides tested (potency: BN = GRP > NMC > NMB, but with similar efficacy: BN = GRP = NMC = NMB). The(More)
The aims of this study were to determine the effect and mechanism of action of pituitary adenylate cyclase-activating peptide (PACAP) on gallbladder muscle. Guinea pig gallbladder muscle strips were studied isometrically. In noncontracted muscle strips, PACAP-27 and PACAP-38 caused dose-dependent contractions, whereas vasoactive intestinal peptide (VIP)(More)
UNLABELLED Pituitary adenylate cyclase activating polypeptide (PACAP) is a neuropeptide with structural homology to vasoactive intestinal polypeptide (VIP). Two receptor types for PACAP have been described: PACAP preferring receptors are selective for PACAP; whereas VIP/PACAP preferring receptors have similar affinity for both PACAP and VIP. Both VIP and(More)
The aims of this study were to determine regional differences and the mechanism of gastric contractile effects of erythromycin. Rabbit gastric circular muscle strips were studied in vitro. The threshold dose for erythromycin was significantly less and the maximum contraction greater in the antrum (1 microM and 0.9 +/- 0.3 kg/cm2) than in the fundus (10(More)