Anthony Madgwick

Learn More
We have assembled the complete protein sequence of the skeletal muscle LIM-protein SLIM by aligning overlapping cDNA sequences. These cDNA sequences were identified from our own sequencing and from BLASTn searches of non-redundant cDNA databases. The predicted SLIM protein sequence included four LIM-domains and a novel single zinc finger domain located in(More)
We have determined the complete mRNA sequence of FHL3 (formerly SLIM2). We have confirmed that it is a member of the family of LIM proteins that share a similar secondary protein structure, renamed as Four-and-a-Half-LIM domain (or FHL) proteins in accordance with this structure. The "half-LIM" domain is a single zinc finger domain that may represent a(More)
OBJECTIVES To assess the extent of lubricant use by smear-takers and the effect of lubricant contamination of ThinPrep processed cervical cytology samples. METHODS All primary care smear-takers were sent a questionnaire on lubricant type and frequency of use. Fifty cervical cytology samples were then contaminated with incremental amounts of K-Y jelly, 50(More)
We have determined the sequence of a novel LIM protein, termed FHL4. It was found to contain the defining secondary structural arrangement of the FHL (formerly SLIM) family of LIM proteins. This sequence was assembled using EST sequences deposited on the dbEST database and from sequencing of PCR fragments, all of which were derived from murine testis cDNA.(More)
Using a cDNA clone derived from a human muscle library we have identified a novel and highly conserved 2.3kb homologue which is highly expressed in skeletal muscle. The partial sequence contains at least three LIM domains and shows greatest homology with the group of LIM-proteins associated with the cytoskeleton and focal adhesion plaques which include(More)
A 40% restriction in maternal feed intake throughout gestation in the guinea-pig results in a 35% reduction in fetal body weight at term and a 20-25% reduction in muscle fibre number. To investigate the effect of maternal undernutrition in early gestation, four nutritional treatments were used: controls-pregnant females fed ad libitum throughout gestation;(More)
To investigate the role of innervation in muscle cell differentiation, the expression pattern of troponin I and troponin T isoforms was investigated in aneural in vivo and in vitro myotubes using immunoblotting and Northern blot hybridisation procedures. The immunoblotting procedure showed that slow troponin I was expressed at high levels in cell cultures(More)
Increased mucosal mass is associated with prostaglandin administration, raising the question as to whether this is the consequence of increased cell production or decreased cell loss. 3H-thymidine was injected into 30 test dogs given 300 micrograms/kg/day of the prostaglandin E1 analogue, misoprostol, orally for 11 weeks, and into 30 dogs which had been(More)
Fetal growth is known to be correlated with the size of the placenta and the exchange surface area. Reduction in the growth of the materno-fetal exchange surface areas may be a mechanism by which the effects of maternal undernutrition on fetal growth are mediated. In the compact placenta of the guinea pig the exchange surface is equivalent to the peripheral(More)
OBJECTIVES To evaluate the potential for machine learning techniques to identify objective criteria for classifying vertical facial deformity. METHODS 19 parameters were determined from 131 lateral skull radiographs. Classifications were induced from raw data with simple visualisation, C5.0 and Kohonen feature maps; and using a Point Distribution Model(More)