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A series of alkylating and aralkylating bromides was used in a comparative study of chemical reactivity (using 4-(p-nitrobenzyl) pyridine as standard nucleophile), and carcinogenic activity (using single injections by the subcutaneous route in 6-week old female CB-hooded rats). Benzyl, ethyl, isopropyl, and trityl bromides were inactive as carcinogens at(More)
Introduction During the 30 years or so following the identification of the first pure chemical carcinogen (1), no common factors or pathways in the mechanism of action of carcinogens from different chemical classes were evident. For this reason perhaps, each class of carcinogen, e.g. the polycyclic aromatic hydrocarbons, the aromatic amines and the(More)
Primary mouse embryo cell cultures were grown in the presence of [14C]guanine, labeling primarily deoxyguanosine residues in the cellular DNA, or in the presence of [14C]adenine, labeling both deoxyguanosine and deoxyadenosine residues in the cellular DNA. These cultures were subsequently exposed to 7,12-[3H]dimethylbenz(a)anthracene for 24 hr. The DNA was(More)
The effect of three reactive potent chemical carcinogens on the passage of MCF-7 cells through the cell cycle was investigated. While these cells, which express wild-type p53, were arrested in G(1) after treatment with actinomycin D (a positive control), treatment with anti-benzo[a]pyrene dihydrodiol epoxide, N-acetoxy-N-2-fluorenylacetamide or(More)
The characterization of eight benzo[a]pyrene-deoxyribonucleoside adducts derived from reaction of the anti-dihydrodiol epoxide and deoxyguanylic and deoxyadenylic acids is described. It is reported that the epoxide ring is opened by the purine amino groups to yield similar amounts of both cis and trans products. NMR data show that the 7- and 8-hydroxyl(More)
7,12-Dimethylbenz[a]anthracene (DMBA)--deoxyribonucleoside adducts formed in mouse skin DNA were quantified in order to determine whether these changed in any systematic fashion under conditions where the tumorigenic activity of DMBA is modified. Similar distributions of adducts were found in male NIH Swiss mice and C57BL mice which exhibit different(More)
To refine our understanding of the mutational spectra one might expect on exposure of human cells to nitric oxide (NO), we have treated the plasmid pSP189 at pH 7.4 with two compounds that generate NO spontaneously in solution, and then sequenced the mutations found when the treated plasmid was transfected into human Ad293 cells and allowed to replicate.(More)