António J. Pereira

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Accurate chromosome segregation during mitosis requires the physical separation of sister chromatids before nuclear envelope reassembly (NER). However, how these two processes are coordinated remains unknown. Here, we identified a conserved feedback control mechanism that delays chromosome decondensation and NER in response to incomplete chromosome(More)
During mitosis, human cells round up, decreasing their adhesion to extracellular substrates. This must be quickly reestablished by poorly understood cytoskeleton remodeling mechanisms that prevent detachment from epithelia, while ensuring the successful completion of cytokinesis. Here we show that the microtubule end-binding (EB) proteins EB1 and EB3 play(More)
The synchronous movement of chromosomes during anaphase ensures their correct inheritance in every cell division. This reflects the uniformity of spindle forces acting on chromosomes and their simultaneous entry into anaphase. Although anaphase onset is controlled by the spindle assembly checkpoint, it remains unknown how spindle forces are uniformly(More)
We investigated the influence of hand posture in handedness recognition, while varying the spatial correspondence between stimulus and response in a modified Simon task. Drawings of the left and right hands were displayed either in a back or palm view while participants discriminated stimulus handedness by pressing either a left or right key with their(More)
Although applicability of kymographs is limited to nearly one-dimensional (1D) processes, they have been instrumental in the analysis and interpretation of a wide range of dynamic biological processes. We focus here on some applications of kymography in the study of one among the range of 'nearly-1D' processes -mitosis. Using this biological context, we(More)
Establishment and maintenance of the mitotic spindle requires the balanced activity of microtubule-associated proteins and motors. In this study we have addressed how the microtubule plus-end tracking protein mast/orbit/CLASP and cytoplasmic dynein regulate this process in Drosophila melanogaster embryos and S2 cells. We show that mast accumulates at(More)
Chromosome positioning at the equator of the mitotic spindle emerges out of a relatively entropic background. At this moment, termed metaphase, all kinetochores have typically captured microtubules leading to satisfaction of the spindle-assembly checkpoint, but the cell does not enter anaphase immediately. The waiting time in metaphase is related to the(More)
The fast rise of the population aging verified in the last decades brings new challenges to the modern societies. Most elderly persons have the usual problems related to the old age, like health chronic problems and sensory and cognitive impairments. Therefore, it becomes essential to ensure the quality of life, safety and well-being to all elderly persons.(More)
The understanding of cytoskeleton dynamics has benefited from the capacity to generate fluorescent fiducial marks on cytoskeleton components. Here we show that light-induced imprinting of three-dimensional (3D) fluorescent speckles significantly improves speckle signal and contrast relative to classic (random) fluorescent speckle microscopy. We predict(More)
Stimulus–response compatibility (SRC) effects are classified depending on the way that the elements of the stimulus and response sets interact, influencing both the speed and accuracy of the motor response. This is particularly important for social stimuli, such as hands. However, the stimuli used in most SRC studies are often simple or abstract figures.(More)
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