António Carvalho Almeida Coimbra

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A significant fall in the number of GABA-immunoreactive cells in laminae I-III of the rat spinal cord occurred in the somatotopic area of projection of the sciatic nerve after nerve transection. The decrease started at 2 weeks post-neurectomy, and at 4 weeks ipsilateral mean cell numbers were approximately 72% of contralateral control values. Similarly, the(More)
Systematic examination of photomontages revealed two types of synaptic glomeruli in laminae II-III. Type I glomeruli have a dark small central (C) terminal of indented contour with closely packed spherical vesicles of variable diameter and few mitochondria. Among the peripheral terminals there are dendritic spines and a few presynaptic dendritic spines (V1(More)
On the basis of dendroarchitecture and cell body shape, complemented with morphometry of dendritic ramification, four major neuronal types were distinguished in lamina I of the spinal cord of the rat. (I) Fusiform spiny cells (39% of impregnated neurons) have longitudinal spindle-shaped perikarya with bipolar, less frequently unipolar, dendritic trees rich(More)
PURPOSE Capsaicin was used to treat symptomatic patients with hyperactive or hypersensitive bladders. MATERIALS AND METHODS Capsaicin solution (1 mM.) was instilled into the bladder of 16 patients with spinal hyperreflexia, bladder instability or hypersensibility. RESULTS Frequency decreased in 14 patients and continence was achieved in 10 of 14(More)
An increase in the number of gamma-aminobutyric acid (GABA)-immunoreactive cells is reported in the superficial dorsal horn of the rat spinal cord upon unilateral inflammation of the hind foot caused by subcutaneous carrageenan injection. The rise of GABAergic cells was restricted to the ipsilateral dorsal horn, reaching a peak value of 23.4% over the(More)
We have shown in previous c-fos studies that noxious stimulation or electroacupuncture in deeply anaesthetized rats activate the hypothalamic pituitary corticotrope axis in a specific way. C-fos expression was more pronounced in the arcuate than the paraventricular hypothalamic nuclei, and none occurred in the pituitary intermediate lobe. The absence of the(More)
The projections of the superficial dorsal horn to the lateral reticular nucleus of the medulla oblongata of the rat, and the morphological types of spinal cord lamina I neurons involved were studied after injecting the retrograde tracer cholera toxin subunit B in the caudal portion of the lateral reticular nucleus. Only injection sites located in the(More)
Fine structural changes were observed in the dark scalloped central C1 terminals of type I synaptic glomeruli in spinal cord segments C6-C7 of the rat 3 days after cutting the three main forelimb nerves. Twenty-six per cent of the C1 terminals occurring on the ipsilateral side showed a lighter appearance due to a decrease in the number of synaptic vesicles.(More)
The nociceptive nature of the neurons of the superficial dorsal horn (laminae I-III) which project to the medullary ventrolateral reticular formation is studied in the rat. Medullary injections of Fluoro-Gold showed exclusive retrograde labeling of laminae I-III cells when the tracer filled a zone intermediate between the lateral tip of the lateral(More)
Retrogradely labelled lamina I neurons were studied after intrathalamic injections of free horseradish peroxidase mixed with dimethylsulphoxide, wheat germ agglutinin conjugated with horseradish peroxidase, and subunit B of cholera toxin. The first two tracers revealed only the perikaryal shape and the orientation of primary dendrites, while cholera toxin(More)