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This unit describes some of the most commonly used yeast vectors, as well as the cloned yeast genes that form the basis for these plasmids. Yeast vectors can be grouped into five general classes, based on their mode of replication in yeast: YIp, YRp, YCp, YEp, and YLp plasmids. With the exception of the YLp plasmids (yeast linear plasmids), all of these… (More)
This unit describes preparation of selected media for growing yeast and also discusses strain storage and revival. Protocols are provided for the assay of beta-galactosidase in liquid culture and for transformation using lithium acetate.
We discuss a family of restricted m–ary partition functions b m,j (n), which is the number of m–ary partitions of n with at most i + j copies of the part m i allowed. We then use generating function dissections to prove the following family of congruences for 1 ≤ t ≤ m − 1 and 2 ≤ k ≤ m − t + 1: