Annette Hamann

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We investigated the correlation between the prevalence of antibodies against human cytomegalovirus (HCMV) "early" proteins and the state of infection. Two hundred and twenty-eight HCMV-IgG-positive (HCMV late-antigen ELISA) sera, drawn from subjects with active and non-active HCMV infections, were tested for specific IgG antibodies against HCMV "early"(More)
We investigated the IgG subclass reactivity pattern to early antigens (EA) of human cytomegalovirus (HCMV) in 217 EA-antibody-positive sera from immunocompetent, healthy persons, renal transplant recipients and AIDS patients by immunoblotting. All IgG subclasses are involved in the IgG immune response to HCMV EA. IgG 1 was the major subclass reacting with(More)
The present retrospective study compares the laboratory diagnosis of cytomegalic inclusion disease (CID) by the use of “shell vial culture” [i.e., immunoperoxidase staining of human cytomegalovirus (HCMV) early antigen in human fibroblasts 24 h postinoculation] to the results of serology (i.e. immunoglobulins IgG, IgM, and IgA HCMV antibody testing) in 21(More)
A new, modular Western blot (immunoblot) system for human immunodeficiency virus (HIV) antibodies (ABN WesPage; Wellcome) was compared with enzyme immunoassays (Wellcome, Behringwerke, and Abbott) and with a U.S. Food and Drug Administration (FDA)-licensed Western blot (DuPont) in a multicenter study. A total of 649 serum samples from HIV patients at(More)
Immunoglobulin A and M patterns to human cytomegalovirus (HCMV) were investigated in sera from actively HCMV infected AIDS patients (n = 61) and healthy HCMV seropositive controls (n = 28) by a Western blot method (modi-blotting) and ELISA. The Western blot showed a higher detection rate for both IgA (78.7% vs. 52.5%) and IgM (83.6% vs. 13.1%) than ELISA in(More)
An antibody capture assay using an enzyme-linked human cytomegalovirus (HCMV) antigen for the detection of specific immunoglobulin E (IgE) was established. IgG, M, and E responses to HCMV were studied in 497 sera obtained from 44 renal transplant recipients and 51 acquired immunodeficiency syndrome (AIDS) patients. The results were compared with those(More)
A significant increase (P = 0.015) in the HIV isolation rate from plasma samples was achieved by use of 10 U/ml exogenous interleukin-2 compared to 20 U/ml. The sensitivity rose from 0% to 29% in patients negative for p24 core antigen (P = 0.031) and from 71% to 86% in patients positive for p24 core antigen in plasma (P > 0.05). Titration of infectious HIV(More)
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