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1. The gated membrane currents (a tetrodotoxin-sensitive Na+ current and a tetraethylammonium- and 4-aminopyridine-sensitive K+ current) of the rapidly adapting stretch receptor neurone of lobster were investigated with respect to their kinetic properties using electrophysiological, pharmacological, and mathematical techniques. 2. The currents were found to(More)
We propose a software architecture for user-cooperative systems, like knowledge systems. This architecture incorporates important notions for building up a context, such as cooperation, explanations and incremental knowledge acquisition. Our main concern in this paper is representation methodology. We show that the architecture can be realized as a pure(More)
Verification of knowledge-bases is a critical step to ensure the quality of a knowledge-based system. The success of these systems depends heavily on how qualitative the knowledge is. Manual verification is however cumbersome and error prone, especially for large knowledge-bases. This thesis provides a design theory, based upon the suggested framework by(More)
1. A polarization-induced membrane current, IQ, was investigated in the slowly adapting lobster stretch receptor neurone using conventional electrophysiological techniques including intracellular ion measurements. 2. The current was readily blocked by Cs+ in a voltage-dependent manner, but proved to be unaffected by tetrodotoxin, tetraethylammonium and(More)
In the lobster stretch receptor neurone it is possible to demonstrate a hyperpolarization-activated membrane current, IQ, which appears to be carried by Na+ and K+ in combination. The ion permeability of the membrane channel conducting this current (Q-channel) was investigated using conventional electrophysiological techniques including intracellular ion(More)
The transmembrane exchange of Na+, K+, and Cl- in slowly and rapidly adapting lobster stretch receptor neurones was studied using ion-sensitive microelectrodes in combination with conventional electrophysiological techniques. The investigation was founded on the assumption that the transmembrane ion exchange is accomplished by active and passive transports(More)
Experiments were performed to identify mechanisms underlying non-leakage and non-H+/HCO3--linked transmembrane Cl- transports in the slowly adapting stretch receptor neurone of the European lobster, using intracellular microelectrode and pharmacological techniques. In methodological tests, it was established that direct estimates of intracellular Cl- with(More)