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We evaluated the neuroprotective effect of MK-801, a noncompetitive, selective N-methyl-D-aspartate receptor antagonist, in a neonatal hypoxic-ischemic animal model. Seven-day-old rats underwent bilateral ligation of the carotid arteries followed by exposure to an 8% oxygen atmosphere for 1 hr. We sacrificed the animals 72 hrs later and assessed the(More)
Nitric oxide synthase (NOS) is present in cultured endothelial cells of cerebrovascular origin in a unique membrane-bound subcellular distribution. The enzyme can be detected by its ability to reduce nitro blue tetrazolium to an insoluble dense blue formazan precipitate. In resting cells, enzyme is located adjacent to the nuclear membrane in a single focus(More)
It is not known if the diabetes-related reduction in blood-brain barrier (BBB) transport of glucose is due to a change in the functional capacity of transporters or to an as yet unidentified mechanism occurring at the plasma membrane or cytoplasm. To increase our understanding of this problem, the cerebral blood flow, the brain uptake index (BUI) of(More)
Measurement of cholinergic muscarinic receptor binding in various rat brain areas using the ligand [3H]quinuclidinyl benzilate indicates that receptor binding is decreased in striatum and cerebellum of aged female rats (22 months old) as compared to younger rats (4 months old). Decreases were not observed in cortex, hippocampus, hypothalamus, or amygdala(More)
To determine the biochemical basis of decreased brain uptake of glucose with age, the brain influx of 3-O-methylglucose (3-O-MG) was measured in male Fischer 344 rats at various ages using the arterial injection-tissue sampling technique of Oldendorf. The Vmax of 3-O-MG transport in the 24-month-old rats (0.22 +/- 0.14 mumol/min/g) was significantly lower(More)
The concentrations of glucose transporter in the cerebral cortex and brainstem of neonatal (4-7 days old) and adult rats were measured using [3H]cytochalasin B binding. There was significantly lower binding in neonatal cortex (1.9 +/- 0.7 pmol/mg protein) compared to adult (8.9 +/- 2.5 pmol/mg protein). Scatchard analysis indicates this difference is due to(More)
Cultured cells derived from the cerebromicrovasculature can be shown to contain the enzyme nitric oxide synthase (NOS) by NADPH diaphorase staining. NOS is located largely as a distinct crescent adjacent to the nuclear membrane. NOS in these cells appears to be associated with the microtubule and microfilament structures and the Golgi apparatus of the cell(More)
[3H]MK-801 binding sites are present in neonate rat brain as early as 3 days after birth. Immature hippocampus and cortex contain approximately one sixth the concentration of binding sites of the adult, while brainstem concentration is twice as high as that of adult. [3H]MK-801 binding is stimulated by glutamate and glycine and blocked by phencyclidine and(More)