Anne M. Hessels

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Zn plays essential and diverse roles in numerous cellular processes. To get a better understanding of intracellular Zn homeostasis and the putative signaling role of Zn, various fluorescent sensors have been developed that allow monitoring of Zn concentrations in single living cells in real time. Thus far, two families of genetically encoded FRET-based Zn(More)
Zinc (Zn2+) ions are increasingly recognized as playing an important role in cellular physiology. Whereas the free Zn2+ concentration in the cytosol has been established to be 0.1-1 nM, the free Zn2+ concentration in subcellular organelles is not well-established. Here, we extend the eCALWY family of genetically encoded Förster Resonance Energy Transfer(More)
Elucidation of subcellular signaling networks by multiparameter imaging is hindered by a lack of sensitive FRET pairs spectrally compatible with the classic CFP/YFP pair. Here, we present a generic strategy to enhance the traditionally poor sensitivity of red FRET sensors by developing self-associating variants of mOrange and mCherry that allow sensors to(More)
Genetically-encoded fluorescent sensor proteins are attractive tools for studying intracellular Zn(2+) homeostasis and signaling. Here we provide an overview of recently developed sensors based on Förster Resonance Energy Transfer (FRET). The pros and cons of the various sensors are discussed with respect to Zn(2+) affinity, dynamic range, intracellular(More)
The Zn(2+)-specific ion channel ZIP7 has been implicated to play an important role in releasing Zn(2+) from the ER. External stimulation of breast cancer cells has been proposed to induce phosphorylation of ZIP7 by CK2α, resulting in ZIP7-mediated Zn(2+) release from the ER into the cytosol. Here, we examined whether changes in cytosolic and ER Zn(2+)(More)
Zn(2+) plays essential and diverse roles in numerous cellular processes. To get a better understanding of intracellular Zn(2+) homeostasis and the putative signaling role of Zn(2+), various fluorescent sensors have been developed that allow monitoring of Zn(2+) concentrations in single living cells in real time. Thus far, two families of genetically encoded(More)
PSR J1023+0038 is an exceptional system for understanding how slowly rotating neutron stars are spun up to millisecond rotational periods through accretion from a companion star. Observed as a radio pulsar from 2007–2013, optical data showed that the system had an accretion disk in 2000/2001. Starting at the end of 2013 June, the radio pulsar has become(More)
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