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The rate at which the acute phase protein response occurred after both major and minor surgery was explored. Increases in the plasma concentration of C-reactive protein (CRP), alpha-1-acid glycoprotein (alpha 1 AG) and fibrinogen were not detected until 6-8 h after the initial incision. The peak concentration of CRP occurred at 48 h and that of fibrinogen(More)
  • A Fleck
  • The Proceedings of the Nutrition Society
  • 1989
1. When assessing the significance of altered plasma protein concentrations the various physiological factors, including posture and hormones which affect the amount and distribution of fluid in the body and the synthesis and catabolism of plasma proteins, must be kept in mind. 2. Although several cytokines, including IL-1 and TNF, are implicated in the(More)
The early time course of the acute phase protein response (APPR) and mediators involved in its control were investigated in the rat and mouse. After turpentine-induced inflammation in the rat C-reactive protein and fibrinogen increased in concentration peaking at 48 h and 18-24 h, respectively. A 9 h delay prior to elevation of these protein was observed.(More)
The development of a sensitive enzyme-linked immunosorbent assay (ELISA) for human metallothionein-1 is reported. Metallothionein was purified from postmortem human liver and used to raise high-titer antibodies in rabbits. The assay was specific for human metallothionein-1 (MT-1), and there was no significant cross-reaction with human metallothionein-2. The(More)
A technique for optimising reagent concentrations on the AutoAnalyzer has been applied to the estimation of ammonia by the Berthelot reaction in the determination of urea and organic nitrogen. Comparison of the use of phenol and salicylate revealed that the optimum concentration of the latter is about four times that of the former. The optimum concentration(More)