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Research on fluorescent semiconductor nanocrystals (also known as quantum dots or qdots) has evolved over the past two decades from electronic materials science to biological applications. We review current approaches to the synthesis, solubilization, and functionalization of qdots and their applications to cell and animal biology. Recent examples of their(More)
Luciferases, which have seen expansive employment as reporter genes in biological research, could also be used in applications where the protein itself is conjugated to ligands to create probes that are appropriate for use in small animal imaging. As the bioluminescence activity of commonly used luciferases is too labile in serum to permit this application,(More)
In this work we analyze the behavior on a company-internal social network site to determine which interaction patterns signal closeness between colleagues. Regression analysis suggests that employee behavior on social network sites (SNSs) reveals information about both professional and personal closeness. While some factors are predictive of general(More)
Teams of specialized experts, such as emergency management planning teams, while making decisions need to efficiently pool domain-specific knowledge, synthesize relevant information, and keep track of collaborators activities at a low interaction cost. This requires tools that allow monitoring both low-level information (e.g., individual actions and(More)
A novel engineered antibody fragment (VL-VH-CH3, or "minibody") with bivalent binding to carcinoembryonic antigen (CEA) was produced by genetic fusion of a T84.66 (anti-CEA) single-chain antibody (scFv) to the human IgG1 CH3 domain. Two designs for the connecting peptide were evaluated. In the T84.66/212 LD minibody, a two-amino acid linker (generated by(More)
In emergency management, collaborative decision-making usually involves collaborative sense-making of diverse information by a group of experts from different knowledge domains, and needs better tools to analyze role-specific information, share and synthesize relevant information, and remain aware of the activities of others. This paper presents our(More)
Establishing and maintaining communication between decision makers, professional responders and general public is vital in many emergency planning and rescue situations. In this paper we present our research on using first-hand information collected by mobile users to increase the flexibility and richness in collaborative emergency management. We propose(More)
PURPOSE The chimeric T84.66 (cT84.66) minibody is a novel engineered antibody construct (V(L)-linker-V(H)-C(H)3; 80 kDa) that demonstrates bivalent and high affinity (4 x 10(10) m(-1)) binding to carcinoembryonic antigen (CEA). The variable regions (V(L) and V(H)) assemble to form the antigen-combining sites, and the protein forms dimers through(More)