Anna Trojak-Goluch

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Interspecific amphihaploid and amphidiploid hybrids between Nicotiana glauca Grah. (2n = 24) and N. tabacum L. (2n = 48) cultivars BY 103 and K 326 were analysed. F1 amphihaploids (2n = 36) were viable and completely self- and cross-sterile, and mostly univalents were present during meiosis (with pairing range from 0 to 5). In some meiocytes, meiotic(More)
Chemically induced polyploids were obtained by the colchicine treatment of shoot tips of Humulus lupulus L. 'Sybilla'. Flow cytometry revealed that most of the treatments resulted in the production of tetraploids. The highest number of tetraploids was obtained when explants were immersed in 0.05% colchicine for 48 h. A field experiment was conducted to(More)
Thielaviopsis basicola and Tomato spotted wilt virus (TSWV) are the most important problems in a moderate climate zone. Previously obtained doubled haploids (DH) of F1 hybrids of the flue-cured line WGL3 resistant to Th. basicola and the dark-cured line PW-834 carrying RTSW-al gene provided the research material. Biological tests and SCAR markers linked(More)
Shoot regeneration was induced after long-term culture of stem- and petiole-derived callus of Humulus lupulus cv. ‘Iunga’. The effects of explant type and plant growth regulators on the regeneration efficiency and polyploid induction were estimated. Callus initiated from stem fragments showed significantly higher shoot regeneration than that obtained from(More)
Black root rot (BRR) caused by Thielaviopsis basicola as well as Tomato spotted wilt virus (TSWV) are the most serious problems in tobacco growing regions. We crossed the breeding line WGL 3 carrying BRR resistance derived from N.glauca with the line PW-834 the resistance of which to TSWV was transferred from cultivar Polalta. Anthers obtained from F(1)(More)
In this study, the morphology, cytology and crossability of amphidiploid hybrids (2n = 8x = 80; WWW′W′TTT′T′) between Nicotiana wuttkei × Nicotiana tabacum were studied. An R1 progeny of the hybrids were produced through in vitro chromosome doubling of the N. wuttkei × N. tabacum cv. Wiślica amphihaploids. The resulting amphidiploids showed enough(More)
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