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An isolated, vascularly perfused preparation of rat intestine extracted large amounts of glutamine (75 pmoles per hour), but no other amino acid, from a recirculated blood perfusate. With L-[UJ%]glutamine, the carbon was partly incorporated into tissue acid-insoluble material (14%) and the rest reappeared with little delay in intestinal venous blood in COZ(More)
The contribution of arterial and luminal substrates as energy sources for small intestine in rats fed a high glucose diet was estimated in vivo, extending previous studies in fasted rats (Windmueller, H. G., and Spaeth, A. E. (1978) J. BioL Chem 253,69-76). Net uptake and release of blood metabolites were measured chemically, and the steady state rates of(More)
Previous work has demonstrated a continuous release of citrulline from the small intestine into the circulation. To evaluate the physiologic significance of this process, we have now measured citrulline uptake and release by isolated, perfused livers and, through surgical means and arteriovenous difference measurements, by various organs of the rat in vivo.(More)
Only a small fraction of the L-[U-14Clglutamate (2%) and the L-[U-14Clglutamine (34%) administered at a 6 rnrw concentration into the lumen of rat jejunal segments in situ was recovered unchanged in venous blood collected from the segments. The remaining 14C of both amino acids was recovered in the blood as CO, (60%), proline (5%), citrulline (4%), alanine(More)
Studies using rat livers perfused with recycled, serum-containing medium plus [3H]leucine revealed that secreted VLDL contain three forms of apolipoprotein B (apoB), B-48, B-95, and B-100, all synthesized by the liver. The B-48/(B-95 + B-100) [3H]leucine incorporation ratio ranged from 0.22 to 3.25 with livers of rats fed different diets, and the ratio was(More)
From measurements of tritium incorporation following the perfusion in situ of livers with blood enriched in 3HZ0, the rate of hepatic fatty acid and cholesterol biosynthesis in young nonfasted rats was found to be 7.0 and 0.09 pmoles per g of liver per hour, respectively. Newly synthesized fatty acids were utilized by liver in the same manner as has been(More)
Obstructive pulmonary disease is a typical feature of cystic fibrosis (CF) and is often associated with bronchial hyperreactivity. Positive skin-test reactions to Aspergillus fumigatus antigens are frequently seen even in nonatopic patients with CF. Full-fledged allergic bronchopulmonary aspergillosis (ABPA) has been estimated to occur in 10% of patients(More)