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Bacterial communities of the water and the biofilm formed during five years on an artificial substrate in Lake Baikal were studied by the pyrosequencing of 16S rRNA gene fragments; taxonomic diversity of bacterial communities and differences in their structure were revealed. The biofilm community contained mainly representatives of three phyla:(More)
Bacterial diversity of two Lake Baikal endemic sponges characterized by different life forms, branching Lubomirskia baicalensis and encrusting Baikalospongia sp., was studied using 454 pyrosequencing of the 16S rRNA gene fragments. In the communities associated with L. baicalensis and Baikalospongia sp., 426 and 428 OTUs, respectively, were identified. In(More)
320 Mass growth of cyanobacteria in various aquatic ecosystems leads not only to technological and aesthetic problems, but also to the contamination of water with substances toxic for humans and animals [1]. Among cyanotoxins, mycrocystins are the best studied; in addition to their toxicity, microcystins have a carcinogenic effect after a long-term action(More)
Microcystis aeruginosa is a well-known Cyanobacterium responsible for the formation of toxic water blooms around the world. Shallow, warm, and eutrophic reservoirs provide the most favourable conditions for M. aeruginosa development. Numerous studies have been devoted to this species, but there still is a necessity to develop additional approaches for the(More)
One of the fundamental methods for cultivating bacterial strains is conventional plating on solid media, but this method does not reveal the true diversity of the bacterial community. In this study, we develop a new technique and introduce a new device we term, I-tip. The I-tip was developed as an in situ cultivation device that allows microorganisms to(More)
A new filamentous cyanobacterial strain BAC 9610 was isolated from the lake Baikal pelagial. Data obtained by light, scanning, and transmission electron microscopy, along with 16S rRNA gene sequence analysis, allowed the bacterium identification as Trichormus variabilis, previously known as Anabaena variabilis. Trichormus is a cyanobacterial genus not(More)
The majority of naturally occurring biofilms contain numerous microorganisms that have not yet been cultured. Additionally, there is little information available regarding the genetic structure and species diversity of these communities. Therefore, we characterised the species diversity, structure and metagenome of biofilms grown on stones and steel plates(More)
Cyanobacteria containing neurotoxic saxitoxin synthesis genes were found in the coastal zone of Lake Baikal near the village of Turka for the first time. Phylogenetic analysis showed that the sequences of saxitoxin synthesis genes belong to the genus Anabaena Bory. Saxitoxin concentration in the water according to ELISA was 1.93 ± 0.64 mg/L. The genetic and(More)
The draft genome sequence of Vibrio cholerae O1 strain I-1263, isolated from a patient in the imported focus in Siberia, was determined. The established structural features of the mobile genetic elements indicate stage-by-stage formation of a highly pathogenic V. cholerae clone and promote understanding of the mechanisms of evolutionary pathogen(More)
Patients with chronic heart failure (CHF) represent a group with high risk of repetitive hospitalizations. In order to assess frequency of repetitive hospitalizations and elucidate their risk factors we included in this study 962 patients with stage IIA-III and functional class II-IV CHF hospitalized in a multiprofile hospital once or repeatedly during 1(More)