Anna Nakayama

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Eukaryotic glycogen debranching enzyme (GDE) possesses two different catalytic activities (oligo-1,4-->1,4-glucantransferase/amylo-1,6-glucosidase) on a single polypeptide chain. To elucidate the structure-function relationship of GDE, the catalytic residues of yeast GDE were determined by site-directed mutagenesis. Asp-535, Glu-564, and Asp-670 on the(More)
Glycogen-debranching enzyme (GDE) gene from Saccharomyces cerevisiae was cloned and expressed into Escherichia coli. A 99.3% homology was found between the nucleotide sequences of GDE gene harbored in the recombinant E. coli plasmid (pTrc99A) and the open reading frame (902039-906646 position) of the 4608-bp fragment of S. cerevisiae chromosome XVI. We(More)
Our design for this project began with building a strong understanding of the current production process. A meeting with the manager, Anna Nakayama, provided a detailed overview of the entire process and outlined some areas of concern. In addition, she provided us with access to critical data such as standard operating procedures and inventory spreadsheets.(More)
An investigation on the applicability of Cartesian grid approach to calculate flow over arbitrary terrain S Vengadesan, A Nakayama & S Yokojima Department of Applied Mechanics, Indian Institute of Technology, Chennai 600 036, India Department of Science of Regional and Built Environments, Graduate School of Science and Technology Kobe University, Kobe 657(More)
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