Learn More
By alloimmunization with whole serum reagents were obtained which revealed two new allotypes, G8 and P30, in pigs. Evidence is presented that the allotypes G8, P30 and previously described G4 and G6 are controlled by three complex autosomal allelic genes forming a closed system. For this new allotype system we propose the designation Gp (globulin, pig). For(More)
Evidence is presented that temperature-sensitive Saccharomyces cerevisiae mutants, impaired in dolichol kinase (Sec59p) or dolichyl phosphate mannose synthase (Dpm1p) activity have an aberrant cell wall composition and ultrastructure. The mutants were oversensitive to Calcofluor white, an agent interacting with the cell wall chitin. In accordance with this,(More)
The association between genes encoding for two serum protein antigens (G9 and G16) and alpha-protease inhibitors was analysed in pigs. A recombination frequency between PI and locus controlling G9 and G16 allotypes varied from 0 to 4.8 cM. Data from appropriate matings are consistent with a gene order of PI1-PO1A-PO1B-PI2-(G9, G16).
Beside the primary illness and environmental factors also alcohol addiction, similarly to another kind of dependency, is being considered as the causative factor of nutritional disorders. Alcohol and drug dependent people due to distorted eating behaviour are predisposed not only to eating disorders (anorexia, bulimia) but also to eating abuse (obesity,(More)
Thermosensitive mutants of Saccharomyces cerevisiae, affected in the endoplasmic reticulum (ER) located glycosylation, i.e. in Dol-P-Man synthase (dpm1), in beta-1,4 mannosyl transferase (alg1) and in alpha-1,3 mannosyltransferase (alg2), were used to assess the role of GDP-Man availability for the synthesis of dolichol-linked saccharides. The mutants were(More)
The aim of the study was the evaluation of the changes in nutritional habits of opiates dependent persons during 4 years of attendance in methadone maintenance treatment conducted by the Department of Toxicology of CMUJ in Kraków. These criteria were fulfilled by 30 persons (23 males and 7 females). They were examined before the methadone treatment and(More)
GDPMan is a key substrate in glycoprotein formation. This is especially true for lower eukaryotes where, in addition to the involvement in N-glycan biosynthesis and GPI-anchor formation, GDPMan takes part in the process which is unique for yeast and fungi i.e. O-mannosylation. Several lines of evidence have been presented that the level of GDPMan affects(More)
Specific alloprecipitins were found in blood plasma of pigs, immunized by sera of Lpr1 positive donors. These precipitins detected a new allotype of the lipoprotein Lpr system which was designated Lpr3. Genetic studies confirmed its codominant inheritance and subgroup character. This linear subgroup of allotype Lpr1 is controlled by the allele Lpr1,3.(More)
In the N-glycosylation reactions catalysed by the Alg enzymes, dolichyl phosphate (DolP) serves as a lipid acceptor of sugar residues. The Alg enzymes are present both on the cytosolic side of the ER: Alg7, Alg1, Alg2 and Alg11) and on its lumenal face(Alg3, Alg5, Alg6, Alg8-10 and Alg12 [3-6]. Cytosolic Alg enzymes, necessary for viability of yeast and(More)