Anna Di Gregorio

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 In this paper we report the cloning, sequence and expression analysis of a new Ciona intestinalis hox gene. On the basis of sequence comparison with mammalian and Amphioxus homologues, we called this gene Cihox5. Northern blot analysis reveals a single transcript of about 1.3 kb in length, that is present from neurula until larva stage. Whole-mount in situ(More)
Several homeobox-containing genes related to Drosophila Distal-less (Dll) have been isolated from a wide variety of organisms and have been shown to function as developmental regulators. While in Drosophila only one Dll gene has been described so far, in Vertebrates many components of the Dlx multigenic family have been characterized. This suggests that,(More)
What Is an Ascidian? Ascidians, or sea squirts, are invertebrate chordates that belong to the earliest branch in the chordate phylum, the subphylum Urochordata or Tunicata (Figure 1A). Ascidian larvae possess a prototypical chordate body plan Joseph C. Corbo,1,3 Anna Di Gregorio,2 and Michael Levine2,3 Department of Pathology Brigham and Women’s Hospital(More)
Ascidians belong to the tunicates, the sister group of vertebrates and are recognized model organisms in the field of embryonic development, regeneration and stem cells. ANISEED is the main information system in the field of ascidian developmental biology. This article reports the development of the system since its initial publication in 2010. Over the(More)
We report the cloning and expression pattern of Ci-msxb the second Ciona intestinalis homeobox gene homologue to the Drosophila muscle segment homeobox (msh) gene. Northern blot analysis showed that transcripts appeared at gastrula stage, peaked in the early tailbud and decreased during the tailed stages. Whole mount in situ hybridization showed that the(More)
Thanks to their transparent and rapidly developing mosaic embryos, ascidians (or sea squirts) have been a model system for embryological studies for over a century. Recently, ascidians have entered the postgenomic era, with the sequencing of the Ciona intestinalis genome and the accumulation of molecular resources that rival those available for fruit flies(More)
Chromatin immunoprecipitation (ChIP) assays allow the efficient characterization of the in vivo occupancy of genomic regions by DNA-binding proteins and thus facilitate the prediction of cis-regulatory sequences in silico and guide their validation in vivo. For these reasons, these assays and their permutations (e.g., ChIP-on-chip and ChIP-sequencing) are(More)
The setting up of the in vitro fertilization (IVF), with embryo-transfer (ET) to the maternal uterus (defined by some groups as embryo-replacement, ER), as a treatment for infertility, foresees a considerable engagement of both human and economic resources. Moreover, the handling of human embryos causes obvious ethical problems. For these reasons, it is(More)
+ These authors contributed equally to the work presented in this paper. 1 Division of Genetics & Development, Department of Molecular and Cell Biology, University of California at Berkeley, Koshland Hall, Berkeley, California 94720. 2 US Department of Energy Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, California, 94598. 3 Department of(More)
Homeobox genes cloned from the purple sea star Pisaster ochraceus (Phylum Echinodermata/Class Asteroidea) were used along with related sequences available from members of other representative animal phyla to generate molecular phylogenies for Distal-less/Dlx, Hox5, Hox7, and Hox9/10 homeobox genes. Phylogenetic relationships were inferred based on the(More)
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