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CD4+-lymphocyte counts (LCs) play a crucial role in the management and monitoring of HIV infection. Variability in CD4+ LCs has been reported to occur as a result of measurement techniques and/or biological variations. We report on the CD4+ LCs of healthy human immunodeficiency virus (HIV)-seronegative adults in Botswana. Samples were obtained from(More)
Background The ability to analyze cryopreserved PBMC for antigen specific T-cells immunity is needed in evaluating response to immune based therapies. Comprehensive studies have demonstrated that the quality of frozen PBMC is critical and maintaining cell viability and functionality by using appropriate cryopreservation techniques is a key to the successful(More)
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