Ann Kathleen Wilson

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OBJECTIVES To review the evidence-based management of nausea and vomiting of pregnancy (NVP) and hyperemesis gravidarum. EVIDENCE MEDLINE and Cochrane database searches were performed using the medical subject headings (MeSH) of treatment, nausea, vomiting, pregnancy, and hyperemesis gravidarum. The quality of evidence reported in these guidelines has(More)
A role for connective tissue growth factor (CTGF) in reproductive function has been suggested from recent studies in the pig. To extend these findings, we have analyzed the immunohistochemical localization of CTGF during the estrous cycle and early pregnancy in mice. During the diestrous and early proestrous stages, CTGF was localized at high levels to both(More)
Myosin II purified from mammalian non-muscle cells is phosphorylated on the 20-kD light chain subunit (MLC20) by the Ca2+/calmodulin-dependent enzyme myosin light chain kinase (MLCK). The importance of MLC20 phosphorylation in regulating cell motility was investigated by introducing either antibodies to MLCK (MK-Ab) or a Ca2+/calmodulin-independent,(More)
Cellular locomotion results from a series of spatially and temporally integrated reactions. The coordinated regulation of these reactions requires sensitive intracellular signaling mechanisms. Because protein phosphorylation reactions represent important signaling mechanisms in mammalian cells, we investigated the effect of okadaic acid, a phosphoprotein(More)
OBJECTIVE To review the clinical aspects of hemorrhagic shock and provide recommendations for therapy. OPTIONS Early recognition of hemorrhagic shock and prompt systematic intervention will help avoid poor outcomes. OUTCOMES Establish guidelines to assist in early recognition of hemorrhagic shock and to conduct resuscitation in an organized and(More)
AIMS To determine the localisation and distribution of connective tissue growth factor (CCN2; CTGF) and transforming growth factor beta type 1 (TGF-beta1) in uterine tissues from cycling and early pregnant pigs. METHODS In situ hybridisation and immunohistochemistry were used to localise CCN2 (CTGF) or TGF-beta1 in uteri obtained from gilts on days 0, 5,(More)
AIMS To determine mechanisms regulating the production of connective tissue growth factor (CCN2; CTGF) and transforming growth factor beta1 (TGF-beta1) in the mouse uterus. METHODS In situ hybridisation and immunohistochemistry were used to localise CCN2 (CTGF) and TGF-beta1 in uteri from sexually mature female mice that had either been (1) mated with(More)
OBJECTIVE To provide guidelines for operative vaginal birth in the management of the second stage of labour. OPTIONS Non-operative techniques, episiotomy, and Caesarean section are compared to operative vaginal birth. outcome: Reduced fetal and maternal morbidity and mortality. EVIDENCE MEDLINE and Cochrane databases were searched using the key words(More)
FIGURE 1. Effect 0fGDPBS, CTP.,S, and GTP on 3H-mucin release. Confluent cultures (16 mm) which had been pretreated with streptolysin 0 were pulsed with H-glucosamine for 24 h and chased for 30 mm in the presence of the nucleotides (200 p.M GTP.,S, 200 iM GDP,,S, and 200 M GTP). In panel A, GDP,,S was added immediately before GTP S. Data represent means ±(More)
Extracellular ATP can stimulate mucin release from primary hamster tracheal surface epithelial (HTSE) cells via a P2 purinoceptor-mediated mechanism, based on agonist potency studies of mucin release (Br. J. Pharmacol. 1991; 103:1053-1056). In the present study, we examined the kinetics of ATP binding to the surface of intact HTSE cells at 4 degrees C using(More)