Ann A. Kiessling

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The reservoir of human immunodeficiency virus (HIV) in semen is unknown. Several lines of evidence suggest that semen HIV may not arise from the same reservoir of infection as peripheral blood. If true, the viral burden in the two compartments could be qualitatively and quantitatively different, a scenario of potentially profound significance for the design(More)
Sperm-mediated DNA transfer to offspring has the potential to markedly simplify the generation of transgenic animals, but the efficiency in mice has been controversial. To determine the basis of the variability of the procedure in mice, we undertook a large, collaborative study of sperm-mediated DNA transfer to mouse eggs in well-established laboratory(More)
OBJECTIVE To test the hypothesis that male reproductive tract leukocytes function in the elimination of abnormal spermatozoa from ejaculated semen. DESIGN Semen specimens with > or = 2 x 10(6) nonspermatozoal cells/mL were examined for leukocytes and for mature sperm with ideal morphology. SETTING Andrology laboratory of a Center of Assisted(More)
The first cleavage of embryos derived from random-bred, inbred, and hybrid-inbred female mice was not arrested by purines at concentrations as high as 30 microM. Development after the first or second cleavage was arrested by hypoxanthine, adenosine or inosine, but not guanosine. In agreement with previous results, the purine-induced block was reversed when(More)
Pronuclear mouse embryos were cultured at four different pHs (7.17, 7.27, 7.37, and 7.50) in Ham's F-10 medium (Gibco, Grand Island, NY) alone and in Ham's F-10 medium supplemented with 10% human cord serum (v/v) to assess the effects of pH and serum on preimplantation embryo development. Media used in these experiments was stored both without (old(More)
Eighteen mature, purebred (Saanen, Alpine, Nubian, La Mancha) dairy does were estrus-regulated with progesterone-impregnated vaginal pessaries. On treatment days 16 to 18, superovulation of 6 does was attempted with pregnant mare's serum gonadotropin (PMSG), and of 12 does with follicle stimulating hormone (FSH). Estrus was induced by progesterone(More)
OBJECTIVES To analyze our natural cycle IVF-ET experience in order to identify parameters predictive for success. DESIGN Retrospective study. SETTING Private-based reproductive center. PATIENTS All patients who presented for IVF-ET interested in unmedicated cycles. MAIN OUTCOME MEASURES Routine monitoring for IVF-ET was performed. In addition, serum(More)
OBJECTIVE To detect and identify bacteria in semen by sequencing polymerase chain reaction (PCR)-amplified ribosomal RNA gene regions (rDNAs). DESIGN Bacterial rDNAs were detected by PCR amplification of semen DNA. Conditions were adjusted to detect only abundant organisms, no fewer than 20,000 bacteria/mL of semen. SETTING Clinical andrology laboratory(More)
Development of zygotes from a hybrid-inbred (B6D2F1) and two random-bred (CD1 and CF1) strains of mice were compared after culture in several modifications of a simple, chemically defined medium based on Earle's Balanced Salts Solution. When cultured without the addition of protein or the chelating agent, ethylenediaminetetraacetic acid (EDTA), none of the(More)