Anjali Chamail

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With the objective of using microtubers for conservation of potato germplasm, the main effects of genotype, abscisic acid (ABA), and sucrose level, and of their interactions on biomass production, microtuberization, microtuber dormancy, and dry matter content, were studied. ABA decreased both microtuber production and microtuber dormancy, whereas higher(More)
In-vitro conservation of microplants at 24±1 °C was investigated in six potato genotypes belonging to different maturity groups. Growth was controlled by using different concentrations of sucrose (20, 40 and 60 g 1−1) alone or in combination with either mannitol (20 and 40 g 1−1) or sorbitol (20 and 40 g 1−1) in Murashige and Skoog medium. Maximum(More)
The popular cloning vector, pUC19, was modified into an in vitro transcription system, pUC-IVT, by incorporation of T7 promoter sequence and appropriate restriction sites for cloning of gene of interest and generation of transcript. Strategy was also devised to eliminate incorporation of undesirable sequences at both 5′ and 3′ ends of the transcript for its(More)
Biotechnology is one of the fastest growing, knowledge-driven industries in India and is expected to play a key role in shaping India’s rapidly developing economy. Since its inception in 1986 the Department of Biotechnology (DBT) has been guiding to foster growth of Indian biotechnology with a range of initiatives. Indian biotechnology industry registered(More)
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