Anita Walker

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A major class of ultraviolet (UV)-resistant derivatives of lex(-) strains of Escherichia coli K-12 grows normally at 30 C but at 42.5 C fails to produce colonies on complete or minimal agar. At 42.5 C these thermosensitive strains form filaments without septa, due to an apparent defect in cell division. Deoxyribonucleic acid degradation in UV-irradiated(More)
It has been shown previously that the radiation sensitivity of lexA- strains of Escherichia coli K-12 can be suppressed by thermosensitive mutations (designated tsl) that are closely linked to the lexA locus and are thought to be intragenic suppressors of lexA mutations (Mount et al., 1973). When a recA mutation is crossed into a suppressed tsl- strain, the(More)
Antibiotics were used to inhibit protein synthesis at specific steps in the biosynthetic pathway. In this way, it was possible to study the coupling of protein synthesis to the accumulation of biologically active mRNA in T4-infected Escherichia coli. Functional mRNA for the phage enzymes deoxynucleotide kinase (EC 2.7.4.4; ATP: nucleoside monophosphate(More)
Host cell reactivation and UV reactivation and mutagenesis of UV-irradiated phage λ were measured in tsl recA + and tsl recA host mutants. Host cell reactivation was slightly more efficient in the tsl recA strain compared to the tsl + recA strain. Phage was UV-reactivated in the tsl recA strain with about one-half the efficiency of that in the wild type(More)
It has been shown previously that the radiation sensitivity of LexA strains of Escherichia coli K-12 can be suppressed by thermosensitive mutations (designated tsl) that are closely linked to the lexA locus. These are thought to be intragenic suppressors that reduce the activity of the diffusible product that gives rise to the LexA- phenotype (Mount et al.,(More)
Antibiotics were used to inhibit protein synthesis at specific steps in the biosynthetic pathway. In this way, it was possible to study the coupling of protein synthesis to the accumulation of biologically active mRNA in T4infected Escherichia coli. Functional mRNA for the phage enzymes deoxynucleotide kinase (EC 2.7.4.4; ATP:nucleosidemonophosphate(More)
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