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The receptor kinase FER is a RALF-regulated scaffold controlling plant immune signaling
TLDR
It is shown that FER acts as a RALF-regulated scaffold that modulates receptor kinase complex assembly, and a similar scaffolding mechanism may underlie FER function in other signaling pathways. Expand
Epigenetic Temporal Control of Mouse Hox Genes in Vivo
www.sciencemag.org (this information is current as of July 6, 2009 ): The following resources related to this article are available online at http://www.sciencemag.org/cgi/content/full/324/5932/1320Expand
McsB Is a Protein Arginine Kinase That Phosphorylates and Inhibits the Heat-Shock Regulator CtsR
TLDR
Structural and biochemical studies show that McsB inhibits DNA binding of CtsR by specifically phosphorylating arginine residues in its DNA binding domain, thereby impairing its function as a repressor of stress response genes. Expand
An extracellular network of Arabidopsis leucine-rich repeat receptor kinases
TLDR
It is shown that CSILRR operates as a unified regulatory network in which the LRR-RKs most crucial for its overall structure are required to prevent the aberrant signalling of receptors that are several network-steps away. Expand
Structural basis for recognizing phosphoarginine and evolving residue-specific protein phosphatases in gram-positive bacteria.
TLDR
The findings uncover the molecular framework for specific targeting of phospho-arginine and suggest that protein arginine (de)phosphorylation may be relevant in eukaryotes. Expand
Molecular features of the UNC-45 chaperone critical for binding and folding muscle myosin
TLDR
In vitro reconstitution and structural biology is used to characterize the interplay between UNC-45 and muscle myosin MHC-B in insect cells and uncover the molecular basis of temperature-sensitive UNC- 45 mutations underlying one of the most prominent motility defects in C. elegans. Expand
GoldenBac: a simple, highly efficient, and widely applicable system for construction of multi-gene expression vectors for use with the baculovirus expression vector system
TLDR
A rapid and simple Golden Gate-based system for the generation of multi-gene expression constructs compatible with baculovirus expression vector systems (BEVS) using either Tn7 transposition or KO1629-based homologous recombination, which is referred to as “GoldenBac”. Expand
The Center for Optimized Structural Studies (COSS) platform for automation in cloning, expression, and purification of single proteins and protein–protein complexes
TLDR
A pipeline is described that enables the systematic screening of expression and purification conditions for recombinant proteins and relies on a series of logical decisions, which allows for easy adoption by any average hypothesis driven laboratory in a manual or robot-assisted manner. Expand
UFD-2 is an adaptor-assisted E3 ligase targeting unfolded proteins
TLDR
In vitro and in vivo experiments are performed and it is shown that UFD-2 has E3 ligase activity and that it ubiquitinates unfolded myos in muscle cells using the C. elegans myosin chaperone UNC-45 as an adaptor protein. Expand
Baculovirus-driven protein expression in insect cells: A benchmarking study.
TLDR
This benchmarking initiative compared the expression and purification results on four selected intracellular proteins and highlighted the significant differences in expression yields obtained using different commercially-packaged baculovirus vectors. Expand
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