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Natural rubber, cis-1,4-polyisoprene, is obtained from a colloidal fluid called latex, which represents the cytoplasmic content of the laticifers of the rubber tree (Hevea brasiliensis). We have developed a method of extracting translatable mRNA from freshly tapped latex. Analysis of in vitro translation products of latex mRNA showed that the encoded(More)
Hevein is a chitin-binding protein that is present in laticifers of the rubber tree (Hevea brasiliensis). A cDNA clone (HEV1) encoding hevein was isolated by using the polymerase chain reaction with mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea latex cDNA library as a template. HEV1 is 1018 base pairs long and includes(More)
Hevea brasiliensis is the major producer of natural rubber which is cis-1,4-polyisoprene. The enzyme 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) is involved in the biosynthesis of rubber and other plant products. We have used a hamster HMGR cDNA clone as a heterologous hybridization probe to isolate and characterize cDNA and genomic clones of(More)
Severa1 homeotic genes that are involved in floral differentiation have recently been isolated and characterized from Antirrhinum majus and Arabidopsis thaliana (Sommer et al., 1990; Yanofsky et al., 1990). Based on genetic and molecular studies, a model has been proposed to explain the roles of different homeotic genes in the specification of floral organ(More)
AIMS To isolate and characterize the endophytes from papaya fruits and to determine the fermentative potential of the strains. METHODS AND RESULTS Endophytes provide potential sources for novel natural products for the use in agriculture and nutrition. There is very limited information on isolation and characterization of bacterial endophytes from papaya.(More)
The fast growing strain, Azorhizobium caulinodans ORS571, isolated from stem nodules of the tropical legume Sesbania rostrata, can grow in the free-living state at the expense of molecular nitrogen. Five point mutants impaired in nitrogen fixation in the free-living state have been complemented by a plasmid containing the cloned fix-ABC region of strain(More)
Commercially used natural rubber (cis-1,4-polyisoprene) is a secondary metabolite of the rubber tree (Hevea brasiliensis). Previous studies have shown the involvement of a prenyl transferase in the final steps of natural rubber biosynthesis which includes polymerization of isopentenyl pyrophosphate into rubber. Using synthetic oligonucleotides corresponding(More)
The latex from Hevea brasiliensis is expelled from specialized cells upon bark tapping. The latex yield is mainly limited by the duration of the latex flow, which is controlled by coagulation processes. Bark treatment with ethylene is known to delay coagulation and increase latex yield. The molecular basis of latex coagulation has been characterized: (1)(More)
In silico docking analysis reported here suggests that insect cellular cytoskeletal beta-actin could be the target of Azadirachtin A (Aza-the principle bioactive compound of neem seeds). The best docking energy of -40.09 kcal/mol at 8.73 A RMSD and predicted hydrogen bond between Arg210 and carboxymethyl group of Aza accompanied with seven hydrophobic(More)
Ribulose-l,5-bisphosphate carboxylase is an enzyme involved in carbon fixation. It is the most abundant protein found in leaf tissue of lightgrown plants. It consists of eight nuclear-encoded small subunits and eight large subunits that are encoded by the chloroplast genome [ 1 ]. We have isolated a cDNA encoding the precursor to the small subunit of(More)