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We previously showed that applying 10(-6) M of the cytokinin 6-benzylaminopurine (BAP) to uninoculated roots of Masym3, a Nod(-)Myc(-)Melilotus alba (Desr.) mutant, resulted in the accumulation of MaENOD40 transcripts to levels similar to BAP-treated wild-type roots. In contrast, inoculation with a Nod(-)S. meliloti mutant expressing the trans-zeatin(More)
Several nonnodulating, nonmycorrhizal (Nod(-)Myc(-)) mutants of Melilotus alba Desr. (white sweetclover) have been described. However, the details of their responses to Sinorhizobium meliloti have not been fully elucidated. We investigated rhizobial entry and colonization using Confocal Scanning Laser Microscopy on the Masym1-5 mutants and isolated an early(More)
The nitrogen-fixing symbiosis between bacteria in the family Rhizobiaceae and members of the legume family (Fabaceae) has been well studied, particularly from the perspective of the early signaling and recognition events. Recent studies of non-nodulating legume mutants have resulted in the identification of a number of genes that are responsive to signal(More)
This paper describes an open-flower mutant, designated opf, that we discovered in a genetic screen of fast neutron bombardment mutants in an attempt at floral-dip transformation of Melilotus alba (Fabaceae; white sweetclover), an alternative papilionoid legume host for Sinorhizobium meliloti. The opf mutant developed flowers with reflexed sepals and petals,(More)
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