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1. Plasma membrane preparations have been isolated from spheroplasts of Saccharomyces cerevisiae, strain R XII, via lysis and subsequent differential centrifugation. These preparations are almost devoid of mitochondria) contamination. 2. The plasma membrane ATPase is fairly stable when refrigerated, but loses activity at 8 °C and above. Below pH 5.6 the(More)
We report on the effect of ethanol and acetaldehyde on yeast chromosomal DNA and on isolated DNA. Ethanol induced DNA single-strand breaks in repair deficient but not in repair proficient Saccharomyces cerevisiae. Acetaldehyde has a deleterious effect on chromosomal DNA in cells as well as on isolated DNA. The results presented support earlier data to show(More)
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