Angela M Tonary

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Hepatitis C virus (HCV) is a global health problem and an important human pathogen. The development of cell culture models for HCV infection has been difficult to accomplish, primarily because HCV is very sensitive to the host cell state. Future models will require the use of three-dimensional (3D) cultures that model the host cell state and environment(More)
The propagation of HCV requires host–virus interactions that support infection, replication, and viral particle assembly. 2] Genotypes 1a and 1b of HCV induce changes in lipid metabolism and cause the formation of endoplasmic reticulum (ER)-derived membranous webs on which HCV replicates. HCV also induces the accumulation of lipid droplets (LDs), known as(More)
BACKGROUND Hepatitis C virus (HCV) infection is a global health problem. A number of studies have implicated a direct role of cellular lipid metabolism in the HCV life cycle and inhibitors of the mevalonate pathway have been demonstrated to result in an antiviral state within the host cell. Transcriptome profiling was conducted on Huh-7 human hepatoma cells(More)
Raman and coherent anti-Stokes Raman scattering (CARS) microscopies have the potential to aid in detailed longitudinal studies of RNA localization. Here, we evaluate the use of carbon-deuterium and benzonitrile functional group labels as contrast agents for vibrational imaging of hepatitis C virus (HCV) replicon RNA. Dynamic light scattering and atomic(More)
A recombinant VH single-domain antibody recognizing staphylococcal protein A was functionalized on reactive lysine residues with N-hydroxysuccimidyl-activated 4-cyanobenzoate. Surface plasmon resonance analysis of antibody-antigen binding revealed that modified and unmodified antibodies bound protein A with similar affinities. Raman imaging of the modified(More)
In this study, a method for the rapid generation of a variety of bifunctional surfaces that can serve to quickly determine the selective adhesion of HEK293 cells towards different chemical functionalities has been established. Using the information about selective adhesion of HEK293 cells to bifunctional surfaces, we demonstrate the ability to construct(More)
C-KIT encodes a tyrosine kinase receptor (KIT) that, when activated by its ligand (KL), stimulates proliferation, differentiation, migration, and survival. Greater than 70% of epithelial ovarian cancers coexpress c-KIT and KL. C-KIT and KL expression levels have been shown to be up-regulated by cAMP in some cell types. Additionally, cAMP is well-recognized(More)
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