Angel Millan

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In this work we are studying whether calcium phosphate deposition (CPD) during vascular calcification is a passive or a cell-mediated mechanism. Passive CPD was studied in fixed vascular smooth muscle cells (VSMC), which calcify faster than live cells in the presence of 1.8 mM Ca²(+) and 2 mM P(i). CPD seems to be a cell-independent process that depends on(More)
Procedures to obtain calcium oxalate monohydrate, dihydrate and trihydrate are presented and discussed. The influence of several additives and conditions in the formation of calcium oxalate dihydrate crystals are compartatively evaluated. It seems that the presence of colloidal phosphate favours the formation of calcium oxalate dihydrate crystals through(More)
BACKGROUND Superparamagnetic iron oxide nanoparticles (SPIONs) are inorganic nanomaterials gaining strong clinical interest due to their increasing number of biological and medical applications. The stabilization of SPIONs in a biocompatible stable suspension (bioferrofluid) is generally achieved by an adequate polymeric coating. As many applications using(More)
A series of maghemite/polymer composite ferrofluids with variable magnetic core size, which show a good efficiency as MRI contrast agents, are presented. These ferrofluids are biocompatible and can be proposed as possible platforms for multifunctional biomedical applications, as they contain anchoring groups for biofunctionalization, can incorporate(More)
Formation of agglomerates of calcium oxalate monohydrate (COM) crystals on semi-batch precipitation performed at conditions relevant to urolithiasis (37 degrees C, pH = 6, initial ratio [Ca]/[Ox] = 10), but without any specific admixture, was followed by both optical and electron microscopy. COM crystals formed on precipitation developed into large(More)
Non-invasive precise thermometers working at the nanoscale with high spatial resolution, where the conventional methods are ineffective, have emerged over the last couple of years as a very active field of research. This has been strongly stimulated by the numerous challenging requests arising from nanotechnology and biomedicine. This critical review offers(More)
Current developments [ 4 ] of thermometers operating below a few micrometers and with high spatial resolution include: Ga-fi lled carbon/MnO nanotubes, [ 2 ] temperature-dependent conformation modifi cations of molecular spring superstructures, [ 3 ] Raman [ 10 ] and infrared [ 11 ] spectroscopic probing, scanning thermal microscopy probes, [ 6 ] and(More)
BACKGROUND Vascular calcification (VC) is primarily studied using cultures of vascular smooth muscle cells. However, the use of very different protocols and extreme conditions can provide findings unrelated to VC. In this work we aimed to determine the critical experimental parameters that affect calcification in vitro and to determine the relevance to(More)
the consciousness of its pathology and physiology and, in turn, on the optimization of therapeutic processes (e.g. in hyperthermal tumour treatment and photodynamic therapy). Conventional temperature sensors are the so-called contact thermometers, in which the measurement of temperature is 25 achieved by heat flow to an invasive probe. 8 The contact nature(More)
This article explores the potential cytotoxic activity of bile-acid cisplatin derivatives like bisursodeoxycholate(ethylenediamine)platinum(II), PtU2, when conjugated with gold nanoparticles, being a promising alternative to cisplatin in the treatment of cancer due to their lower toxicity. For our purpose we analyzed the intracellular delivery ability of(More)