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The responsiveness of central noradrenergic systems to stressors and cocaine poses norepinephrine as a potential common mechanism through which drug re-exposure and stressful stimuli promote relapse. This study investigated the role of noradrenergic systems in the reinstatement of extinguished cocaine-induced conditioned place preference by cocaine and(More)
BACKGROUND Although allergen-specific IgE content in serum can be determined immunochemically, little is known about the relationship between this parameter and the strength of the degranulation response upon allergen triggering. OBJECTIVES Analyse the degranulation capacity of immunochemically defined purified and serum IgE after challenge with anti-IgE(More)
Alveolar macrophages and mast cells isolated from guinea pig lung were passively sensitized with IgG1, IgG2, or serum obtained from guinea pigs actively sensitized with ovalbumin. The release of histamine by mast cells and of thromboxane A2 by alveolar macrophages upon ovalbumin challenge indicated that both antibodies and serum were capable of sensitizing(More)
Histamine release experiments were performed over a 10(6)-fold range of allergen concentrations on basophils of patients sensitive to Dermatophagoides farinae. Two extracts of D. farinae were used, a partially purified one (Df 80d) containing several allergens and a highly purified component (Ag11). This histamine release patterns obtained with the complex(More)
BACKGROUND Allergens from the house dust mite Dermatophagoides farinae are responsible for frequent respiratory allergic disorders, but only 3 groups of these allergens are well characterized. OBJECTIVE This study was performed to complete the repertoire of D farinae allergens using two-dimensional (2-D) electrophoresis. METHODS D farinae mite(More)
Birch pollen allergy is a very frequent pathology in Europe and North America. More than 95% of the tree pollen allergic patients display IgE reactivity against Bet v 1, the major birch pollen allergen. Starting with PBL from a patient desensitized by immunotherapy, we have generated five B cell lines (BAB1 to BAB5) that secrete human IgG mAbs of high(More)
We report the molecular characterization of five human monoclonal antibodies, BAB1-5 (BAB1: IgG(1); BAB4: IgG(2); BAB2, 3, 5: IgG(4)), with specificity for the major birch pollen allergen, Bet v 1. BAB1-5 were obtained after immunotherapy and contained a high degree of somatic mutations indicative of an antigen-driven affinity maturation process. While BAB1(More)
Basophils from allergic or non-atopic donors were depleted for their native membrane IgE by acid treatment and then passively sensitized by Dermatophagoïdes farinae specific IgE containing sera. Histamine release experiments were performed with a highly purified allergen (Df 11) on native cells, acid treated cells and passively sensitized cells. In the(More)
The distributions of histamine and mast cells in the intestinal mucosa have been studied in conventional (CV) and germ-free (GF) rats. Both distributions do not appear to be uniform throughout the gastrointestinal tract. The presence of digestive microflora influences these distributions: more histamine and mucosal mast cells are present in the small(More)
OBJECTIVE In the present study, we investigated signal transduction pathways involved in TNF-alpha gene expression and TNF-alpha secretion by mast cells stimulated through the high affinity IgE receptor (FcepsilonRI). MATERIALS AND METHODS TNF-alpha mRNA steady state levels and TNF-alpha secretion in the presence of specific pharmacological agents were(More)