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A gas-liquid chromatographic-mass spectrometric (GLC-MS) method was applied to the detection of 3-deoxy-d-manno-2-octulosonic acid (Kdo), a constituent of bacterial lipopolysaccharide (LPS, endotoxin). Samples containing LPS were dried, methanolyzed with 2 M HCl in methanol at 60 degrees C for 1 h and acetylated with acetic anhydride and pyridine (1:1, v/v)(More)
Structural studies on the major glycolipid isolated from Rothia mucilaginosa were carried out utilising specific chemical degradation, NMR spectroscopy and matrix-assisted laser-desorption/ionization time of flight mass spectrometry (MALDI TOF-MS). The glycolipid was found to be a dimannosylacylmonoglyceride in which the carbohydrate part was the(More)
A Gram-positive bacterium, strain J18(T), isolated from sludge of a foul water sewer, was subjected to a polyphasic taxonomic study. Phylogenetic analysis of the bacterium based on its 16S rDNA sequence showed that it belongs to the genus Rothia and forms a distinct phyletic clade with the type strain of Rothia nasimurium. Morphological, physiological and(More)
Lipopolysaccharides with sialic acid as a component were isolated from six bacterial serovars of the family Enterobacteriaceae. SDS-polyacrylamide gel electrophoresis, immunoblotting and passive haemagglutination demonstrated that epitopes are localized in the O-specific polysaccharide of lipopolysaccharides. The strains were tested for a serological(More)
The aim of this study was to evaluate the cytotoxicity of dried aqueous extracts from Thymus serpyllum (ExTs), Thymus vulgaris (ExTv), Majorana hortensis (ExMh), and Mentha piperita (ExMp), and the phenolic compounds caffeic acid (CA), rosmarinic acid (RA), lithospermic acid (LA), luteolin-7-O-glucuronide (Lgr), luteolin-7-O-rutinoside (Lr),(More)
The chemical structure of the major glycolipid present in the chloroform-methanol extract of bacterial biomass of Tsukamurella pulmonis is reported. This compound was purified by TLC and HPLC. The sugar analysis revealed only glucose. Detailed chemical analyses, NMR, and MALDI FT-ICR-mass spectrometric studies identified(More)
Antibodies induced in mice by the N-propionyl (N-Pr)-group B meningococcal polysaccharide (GBMP)-tetanus toxoid (TT) conjugate were bactericidal for GBM organisms independent of protein serotype. The antisera contained two populations of N-Pr-GBMP-specific antibodies, only one of which crossreacted with the GBMP. Particularly significant was the fact that(More)
The lipopolysaccharides from Escherichia coli O24 and O56 could be separated into higher-molecular-mass and lower-molecular-mass fractions. Mild acid hydrolysis of lipopolysaccharides of both serotypes released an O-specific polysaccharide and a tetrasaccharide repeating unit. Oligomers of the repeating unit, the core and the oligosaccharide that contains a(More)
We investigated the action of the quaternary ammonium salt (QAS) called IM (N-(dodecyloxycarboxymethyl)-N,N,N-trimethyl ammonium chloride) on Saccharomyces cerevisiae yeast cells. Changes in the yeast cell ultrastructure were confirmed by electron microscopy. We treated resistant mutant cells with QAS, and confirmed destruction of the mutant cytoplasm, an(More)
The N-propionylated group B meningococcal polysaccharide mimics a unique bactericidal epitope on the surface of group B meningococci and Escherichia coli K1. This was confirmed when both the above organisms were able to absorb the bactericidal antibodies from a mouse-anti-N-propionylated group B meningococcal polysaccharide-tetanus toxoid conjugate serum.(More)