Andrew R. Ready

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Foreign tissues grafted into healthy recipients are usually rejected by the hosts' immune system largely by means of major histocompatibility complex (MHC) products expressed on donor cells. During ontogeny, developing T lymphocytes acquire tolerance to self-MHC antigens and the thymus has been considered as the most likely site for the abrogation of(More)
Visualizing the three-dimensional morphology and spatial patterning of cells embedded deep within dense connective tissues of the musculoskeletal system has been possible only by utilizing destructive techniques. Here we utilize fructose-based clearing solutions to image cell connectivity and deep tissue-scale patterning in situ by standard confocal(More)
INTRODUCTION Calciphylaxis occurring after kidney transplantation is rare and rarely reported. It results in chronic non-healing wounds and is associated with a poor prognosis and is often fatal. We present a case of proximal lower limb calciphylaxis that occurred early after kidney transplantation. The patient had no classic associated risk factors. He had(More)
INTRODUCTION Hypothermic machine perfusion offers great promise in kidney transplantation and experimental studies are needed to establish the optimal conditions for this to occur. Pig kidneys are considered to be a good model for this purpose and share many properties with human organs. However it is not established whether the metabolism of pig kidneys in(More)
BACKGROUND The aim of this study is to determine the feasibility of using nuclear magnetic resonance (NMR) tracer studies ((13)C-enriched glucose) to detect ex vivo de novo metabolism in the perfusion fluid and cortical tissue of porcine kidneys during hypothermic machine perfusion (HMP). METHODS Porcine kidneys (n = 6) were subjected to 24 h of HMP using(More)
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