Andrew G. Ulsamer

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The lipids of Acanthamoeba castellanii (Neff) consist of 52% neutral lipids and 48% polar lipids. Triglycerides account for 75% and free sterols for 17% of the neutral lipids. The major phospholipids are phosphatidylcholine (45%), phosphatidylethanolamine (33%), phosphatidylserine (10%), a phosphoinositide (6%), and diphosphatidylglycerol (4%). The(More)
Plasma membranes were isolated from the ameba Acanthamoeba castellanii by low-speed velocity centrifugation followed by equilibrium centrifugation in a sucrose gradient. The isolated membranes had a high ratio of sterol to phospholipid (0.98 moles/mole) and of phospholipid to protein (0.43 mg/mg). The plasma membranes had very low concentrations of DNA,(More)
Exposure to formaldehyde appears to be associated with hepatoxicity in many species, including humans, following injection, ingestion, or inhalation. Macroscopic, microscopic, and biochemical manifestations in the liver include alterations in weight, centrilobular vacuolization, focal cellular necrosis, and increased alkaline phosphatase concentrations.(More)
Carbon-14-labeled formaldehyde was used per se, or was used in the synthesis of dimethyloldihydroxyethyleneurea (DMDHEU), which was incorporated into cotton or cotton/polyester blend fabric. Patches of the fabric containing known quantities of radioactive DMDHEU were applied to the backs of New Zealand White rabbits for periods up to 48 h. The rabbits were(More)
Surface tris-(2,3-dibromopropyl) phosphate (TRIS) was removed from flame-retarded polyester fabric by benzene-hexane extraction and replaced with 14C-TRIS. Sections of the radiolabeled fabric (10 X 12 cm) were placed in contact with the clipped skins of rabbits, and urine and feces were collected over a 96 hr period. The cloths were allowed to remain dry or(More)
Biochemical response to the toxic lung damage induced by inhalation of methylene chloride was studied. Significant increases in protein, hexose, sialic acid, lactate dehydrogenase, acid and alkaline phosphatase content were observed in the cell-free lavage effluents from lungs of exposed rats compared to the control animals. This was interpreted as(More)