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SmartFrog is a framework for creating configuration-driven systems. It has been designed with the express purpose of making the design, deployment and management of distributed component-based systems simpler and more robust. Over the last decade it has been the focus for ongoing research into aspects of configuration management and large-scale distributed(More)
Cloud-based MapReduce services process large datasets in the cloud, significantly reducing users' infrastructure requirements. Almost all of these services are cloud-vendor-specific and thus internally designed within their own cloud infrastructures, resulting in two important limitations. First, cloud vendors don't let developers see and evaluate how the(More)
The simultaneous targeting of host and pathogen processes represents an untapped approach for the treatment of intracellular infections. Hypoxia-inducible factor-1 (HIF-1) is a host cell transcription factor that is activated by and required for the growth of the intracellular protozoan parasite Toxoplasma gondii at physiological oxygen levels. Parasite(More)
Exocytosis is essential to the lytic cycle of apicomplexan parasites and required for the pathogenesis of toxoplasmosis and malaria. DOC2 proteins recruit the membrane fusion machinery required for exocytosis in a Ca(2+)-dependent fashion. Here, the phenotype of a Toxoplasma gondii conditional mutant impaired in host cell invasion and egress was pinpointed(More)
Next generation sequencing is helping to overcome limitations in organisms less accessible to classical or reverse genetic methods by facilitating whole genome mutational analysis studies. One traditionally intractable group, the Apicomplexa, contains several important pathogenic protozoan parasites, including the Plasmodium species that cause malaria. Here(More)
Cloud computing infrastructure services enable the flexible creation of virtual infrastructures on-demand. However, the creation of infrastructures is only a part of the process for provisioning services. Other steps such as installation, deployment, configuration, monitoring and management of software components are needed to fully provide services to(More)
Toxoplasma gondii has a largely clonal population in North America and Europe, with types I, II and III clonal lineages accounting for the majority of strains isolated from patients. RH, a particular type I strain, is most frequently used to characterize Toxoplasma biology. However, compared to other type I strains, RH has unique characteristics such as(More)
Deidentified newborn screening bloodspot samples (NBS) represent a valuable potential resource for genomic research if impediments to whole exome sequencing of NBS deoxyribonucleic acid (DNA), including the small amount of genomic DNA in NBS material, can be overcome. For instance, genomic analysis of NBS could be used to define allele frequencies of(More)