Andrew C. Butcher

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An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of bovine herpesvirus type 1 (BHV-1) antigens in nasal swab specimens collected from infected animals. Development of the ELISA involved screening and selection of BHV-1-specific monoclonal antibodies for their ability to capture BHV-1 antigens and for their stability and activity(More)
The virus specificity of antibodies against bovine herpes virus type 1 was determined with a radioimmunoprecipitation assay and serum collected from natural and experimentally induced infections. By using sequentially collected sera, the development of antibodies to 4 to 5 viral glycoproteins and 11 to 12 nonglycosylated proteins was followed for the first(More)
Three serological assays were compared for detection of antibodies to bovine herpes-virus type 1. These were virus neutralization (VN), enhanced complement fixation (CF) and enzyme-linked immunosorbent assay (ELISA). The ELISA was developed using an infected cell lysate antigen and purified virus and was optimized in relation to antigen and antisera(More)
Monoclonal antibodies were used to study neutralizing determinants on polypeptides of bovine herpesvirus 1. Two of three monoclonal antibodies which recognized nonoverlapping epitopes on a glycoprotein of 82,000 daltons were found to neutralize. A second group of monoclonal antibodies that individually precipitated five viral glycopolypeptides ranging in(More)
IT has often been assumed that in the homothallic Ascomycete Aspergillus nidulans, heterokaryon formation, the bringing together of unlike nuclei into the same cytoplasm, is a prerequisite of both parasexual and sexual recombination (Pontecorvo et al., 1953; Grindle, 1963b; Jinks and Grindle, 1963; Caten and Jinks, 1966). Grindle has shown, however (l963a(More)
WILD isolates of the fungus Aspergillus nidulans can be classified into clearly defined heterokaryon compatibility (h-c) groups (Grindle, 1 963a, b). Heterokaryon formation between pairs of wild isolates is restricted to members of a single h-c group. All pairwise combinations of isolates from within a group thus readily form heterokaryotic conidial heads(More)
Sea spray particles ejected as a result of bubbles bursting from artificial seawater containing salt and organic matter in a stainless steel tank were sampled for size distribution, morphology, and cloud condensation nucleus (CCN) activity. Bubbles were generated either by aeration through a diffuser or by water jet impingement on the seawater surface.(More)
HETEROKARYOSIS, the bringing together of unlike nuclei into the same cytoplasm, has been demonstrated in many fungi (see Davis, 1966; Caten and Jinks, 1966). In fungi imperfecti it is an essential step in the parasexual cycle, which is the sole means whereby genetic exchange can be achieved. In these fungi, therefore, heterokaryon incompatibility, as(More)
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