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Hydrogen sulfide (H2S) is a widespread gasotransmitter also known as a powerful neuroprotective agent in the central nervous system. However, the action of H2S in peripheral synapses is much less studied. In the current project we studied the modulatory effects of the H2S donor sodium hydrosulfide (NaHS) on synaptic transmission in the mouse neuromuscular(More)
In our experiments on motor nerve endings of the frog cutaneous pectoris muscle, using fluorescent marker FM 1-43, the intensity and topography of endocytosis were investigated after the initiation of massive exocytosis of synaptic vesicles by increasing the extracellular potassium concentration. Using FM 1-43, fluorescent spots were shown to appear,(More)
The amplitude-temporal characteristics of miniature end-plate currents (MEPC) were studied in denervated frog cutaneous-pectoral muscles. After the nerve cutting, the initial shortening of MEPC decay time was followed by its prolongation. In 6-8 days after the denervation, the MEPC sensitivity to membrane potential decreased, while the average open time of(More)
In experiments on neuromuscular preparations of the frog sartorius muscle the evoked transmitter release was investigated by extracellular recording. A decrease of the evoked transmitter release from proximal to distal parts of nerve terminals was observed related to a decrease in probability of transmitter quantum release. The terminal part of synapse(More)
During normal cell metabolism the production of intracellular ATP is associated with the generation of reactive oxygen species (ROS), which appear to be important signalling molecules. Both ATP and ROS can be released extracellularly by skeletal muscle during intense activity. Using voltage clamp recording combined with imaging and biochemical assay of ROS,(More)
Exogenous adenosine triphosphoric acid produces a biphasic effect on the resting membrane potential of muscle fibers in rat diaphragm. Depolarization of the sarcolemma observed 10 min after application of adenosine triphosphoric acid results from activation of Na+/K+/2Cl− cotransport. The increase in chloride cotransport is related to activation of(More)
The review is devoted to exposition of a physiological role of a nitric oxide (NO), free radical gas, in various physiological functions. The number of those NO involvements is extremely high: bacteriocidal, cytotoxic and antitumor leukocyte effects, a relaxation of smooth-muscle cells of both vessels and gastrointestinal tract, the name just a few. The(More)
The histochemical and electrophysiological investigation of tonic muscle fibers was performed in the frog m. cruralis. Cholinesterase activity studies in tonic fibers revealed 4 to 14 synapses aggregated in groups. A number of synapses were in positive correlation with the muscle fibers length. In MPSP recording, the distribution of amplitude, periods of(More)
Nitric oxide is the first gaseous messenger whose functions were comprehensively studied in different systems of organism. Recently, new data on the physiological role of other endogenous gases: carbon monoxide and hydrogen sulfide, appeared. The role of gases in gastrointestinal tract and cardiovascular system have been established; however, data on their(More)
In the present study we combined FM 1-43 imaging and electrophysiological recording of miniature end-plate currents (MEPCs) to determine the role of extracellular calcium in synaptic vesicle exo- and endocytosis at the frog motor nerve terminals. We replaced extracellular Ca2+ ions with other bivalent cations (Sr2+, Ba2+, Cd2+, Mg2+) or used a calcium-free(More)