Andreas Kühn

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Shotgun proteomics is a powerful technology for global analysis of proteins and their post-translational modifications. Here, we investigate the faster sequencing speed of the latest Q Exactive HF mass spectrometer, which features an ultra-high-field Orbitrap mass analyzer. Proteome coverage is evaluated by four different acquisition methods and benchmarked(More)
During the last decades, molecular sciences revolutionized biomedical research and gave rise to the biotechnology industry. During the next decades, the application of the quantitative sciences--informatics, physics, chemistry, and engineering--to biomedical research brings about the next revolution that will improve human healthcare and certainly create(More)
The quadrupole Orbitrap mass spectrometer (Q Exactive) made a powerful proteomics instrument available in a benchtop format. It significantly boosted the number of proteins analyzable per hour and has now evolved into a proteomics analysis workhorse for many laboratories. Here we describe the Q Exactive Plus and Q Exactive HF mass spectrometers, which(More)
The quantitative analysis of protein mixtures is pivotal for the understanding of variations in the proteome of living systems. Therefore, approaches have been recently devised that generally allow the relative quantitative analysis of peptides and proteins. Here we present proof of concept of the new metal-coded affinity tag (MeCAT) technique, which(More)
In mass spectrometry-based proteomics, data-independent acquisition (DIA) strategies can acquire a single data set useful for both identification and quantification of detectable peptides in a complex mixture. However, DIA data are noisy owing to a typical five- to tenfold reduction in precursor selectivity compared to data obtained with data-dependent(More)
When a disaster occurs, the rapid gathering and sharing of crucial information among public safety agencies, emergency response units, and the public can save lives and reduce the scope of the problem; yet, this is seldom achieved. The lack of interoperability hinders effective collaboration across organizational and jurisdictional boundaries. In this(More)
IT-Erfolgsfaktoren beschreiben Bedingungen, die maβgeblich zur erfolgreichen Umsetzung von IT-Projekten beitragen. Über das IT-Risikomanagement wird dabei präventiv versucht, kritischen Erfolgsfaktoren Rechnung zu tragen, was u.a. durch den Einsatz von IT-Governance-Methoden unterstützt und geprüft werden kann. Der vorliegende Beitrag setzt sich mit der(More)
Garbage-Collection for reconfigurable hardware systems is another step to make dynamic reconfigurable hardware usable. By now, dynamic reconfiguration of FPGAs is still based on common hardware design concepts. This work describes a garbage-collector implementation for a reconfigurable hardware system, as part of a more dynamic hardware design concept. In(More)