Andrea Picco

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Correlative electron and fluorescence microscopy has the potential to elucidate the ultrastructural details of dynamic and rare cellular events, but has been limited by low precision and sensitivity. Here we present a method for direct mapping of signals originating from ∼20 fluorescent protein molecules to 3D electron tomograms with a precision of less(More)
In this paper we consider the modeling of a selected portion of signal transduction events involved in the angiogenesis process. The detailed model of this process contains a large number of parameters and the data available from wet-lab experiments are not sufficient to obtain reliable estimates for all of them. To overcome this problem, we suggest ways to(More)
In this paper we consider the modeling of a portion of the signal transduction pathway involved in the angiogenic process. The detailed model of this process is affected by a high level of complexity due to the functional properties that are represented and the size of its state space. To overcome these problems, we suggest approaches to simplify the(More)
Clathrin-mediated endocytosis is an essential process that forms vesicles from the plasma membrane. Although most of the protein components of the endocytic protein machinery have been thoroughly characterized, their organization at the endocytic site is poorly understood. We developed a fluorescence microscopy method to track the average positions of yeast(More)
In a previous paper (Picco et al., 2015), the dynamic architecture of the protein machinery during clathrin-mediated endocytosis was visualized using a new live imaging and particle tracking method. Here, by combining this approach with correlative light and electron microscopy, we address the role of clathrin in this process. During endocytosis, clathrin(More)
In this work, we present an experimental procedure to measure the composition distribution within inhomogeneous SiGe nanostructures. The method is based on the Raman spectra of the nanostructures, quantitatively analyzed through the knowledge of the scattering efficiency of SiGe as a function of composition and excitation wavelength. The accuracy of the(More)
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