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Antiviral drugs have demonstrated significant therapeutic potential against phytoviruses, such as inosine monophosphate dehydrogenase inhibitors like mycophenolic acid or thiopurines. However, drug delivery across cellular barriers is a challenging task that calls for investigation. In this study, membrane transport of three antiviral drugs such as(More)
Food contamination by toxic pesticides has induced intense research for alternative methods to control pests and diseases. For instance, the phasing out of methyl bromide used for soil disinfection has led to a reconsideration of heat-based methods to control soil-borne pathogens. Techniques such as soil steaming and soil solarization have been applied(More)
A traceable declaration of health is now necessary for many plants, especially those being monitored for disease such as certified nursery stock. Radio-frequency identification (RFID) micro-chips placed in woody plants can be used to store and retrieve information on their health status through all phases of propagation and in the field. The microchip is(More)
Automation in agriculture should improve plant health, product quality, and production efficiency. However the actual use of electronic identification tools in agriculture is limited. Therefore, I review here electronic identification applications to support plant health and production and agricultural sustainability. The major points are as follows: (1)(More)
Electrophysiological techniques were applied to investigate the action of antiviral drugs during trans-plasma events in in vivo grapevine cells infected by GLRaV-1 and GLRaV-3. Carbon fiber microelectrodes and redox-sensitive dyes were used to measure trans-plasma membrane electron transport (t-PMET) activity in healthy and infected samples treated with(More)
In many European rural areas, agriculture is not only an economic activity, but it is strictly linked to environmental and social characteristics of the area. Thus, sometimes, a pathogen can become a social threat, as in the case of Xylella fastidiosa and olive trees (Olea europaea L.) in Salento. Fast and systemic response to threats represents the key to(More)
To evaluate the role of purines in antiviral treatments in plants, ribavirin (RB) and tiazofurin (TZ) were applied in combination with guanosine (GS) or adenosine (AS) in in vitro grapevine or tobacco explants infected by Grapevine leafroll associated virus 3 (GLRaV-3) and Cucumber mosaic virus (CMV), respectively. Using a microelectrochemical (trans-plasma(More)
The trans-plasma membrane behavior in virus-infected grapevine leaves was investigated and the effects of six viruses included in European and Italian certification protocols of grapevine on trans-plasma membrane potential (t-PMEP) or electron transport (t-PMET) activity were evaluated. Electrophysiological tests were carried out on leaf samples of(More)
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