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The responses of cultured chick embryo retinal neurons to several extracellular matrix molecules are described. Retinal cell suspensions in serum-free medium containing the "N1" supplement (J. E. Bottenstein, S. D. Skaper, S. Varon, and J. Sato, 1980, Exp. Cell Res. 125, 183-190) were seeded on tissue culture plastic surfaces pretreated with polyornithine(More)
It is well established that many types of cells are dependent on trophic factors for their survival. We are investigating whether photoreceptor survival may also be dependent upon such a factor, as well as the possibility that the interphotoreceptor matrix (IPM), which lies between the outer retina and the RPE, may be a source of photoreceptor(More)
Bruch's membrane may provide a selective filtration barrier for nutrients coming from the choriocapillaris to the outer retina. Because proteoglycans have been shown to have structural and filtration properties in other tissues, we have been investigating the deposition of newly synthesized proteoglycans into Bruch's membrane and how these may be affected(More)
Macular degeneration associated with age and drusen, an important cause of visual loss, is associated clinically with alterations in the retinal pigmented epithelium. Because the pigmented epithelium is a copper-rich tissue with antioxidant properties, the copper economy in patients and controls were studied by measuring ceruloplasmin. Ceruloplasmin, a(More)
Epiretinal membrane formation in the posterior pole is an almost constant feature of eyes with retinitis pigmentosa. Using a modified direct immunofluorescence reaction, we demonstrated a strong reaction with antilaminin in a band-like pattern on the inner retinal surface of 3 eyes with retinitis pigmentosa. There were also strongly-laminin-positive(More)
1. In recent years, a number of histochemical and immunocytochemical studies have suggested that proteoglycans, particularly those in the interphotoreceptor matrix, exhibit altered distributions in several murine models for retinal degenerations. We are using a cell culture system to characterize the proteoglycans synthesized by neurons and photoreceptors(More)
The Pew Home Visiting Campaign partners with policy makers and advocates to promote smart state investments in quality, voluntary home-based programs for new and expectant families. The Pew Center on the States is a division of The Pew Charitable Trusts that identifies and advances effective solutions to critical issues facing states. Pew is a nonprofit(More)
It has been demonstrated that differences exist in the lateral mobility of Con A- and WGA-binding sites in the membranes of normal and brachypod mouse limb mesenchymal cells (Hewitt et al., 1978). The work presented here investigates the involvement of microtubules and microfilaments as mediators of binding site mobility in this system. Treatment of cells(More)
Proteoglycans are involved in a variety of cell-cell and cell-matrix interactions. These include cell adhesion, growth regulation and a number of developmental processes. Their involvement in such interactions may be of particular importance in retinitis pigmentosa (RP) because of the detachment and migration of retinal pigment epithelial (RPE) cells often(More)
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