Andrea Barretto Motoyama

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AU-rich elements (AREs) in the 3' untranslated region (UTR) of unstable mRNAs dictate their degradation. An RNAi-based screen performed in Drosophila S2 cells has revealed that Dicer1, Argonaute1 (Ago1) and Ago2, components involved in microRNA (miRNA) processing and function, are required for the rapid decay of mRNA containing AREs of tumor necrosis(More)
Acute lymphoblastic leukemia (ALL) is the most common childhood malignancy. Although several clinical characteristics can be associated with worse prognosis, more robust biological markers still remains uncovered. SMYD2, a member of SMYD protein family, regulates the activity of several proteins through methylation. In this study, we performed quantitative(More)
The PR-like proteins, class I β-1,3-glucanase (GLU I) and chitinase (CHN I), are induced as part of a stereotypic response that can provide protection against viral, bacterial, and fungal pathogens. We have identified two Nicotiana plumbaginifolia ankyrin-repeat proteins, designated $$\underline{G}$$ lucanohydrolase $$\underline{B}$$ inding(More)
We have amplified and sequenced the 5.8S and 28S ribosomal DNA genes and intergenic regions of Paracoccidioides brasiliensis, strain Pb01. Using primers specifically designed for both ribosomal DNA regions, we were able to discriminate between P. brasiliensis and other human pathogenic fungi by PCR. The use of this molecular marker could be important for(More)
OBJECTIVES The objective of this study was to evaluate the association between estimated human papillomavirus (HPV) viral load and abnormal cytology on anal samples. METHODS Anal cytological samples of 42 HIV-positive patients were analysed by conventional cytology and Hybrid Capture II. RESULTS On cytology, 30.95% (13 of 42) anal samples were positive(More)
Search of the Genes that Are Required for ARE-Mediated mRNA Decay in Drosophila S2 Cells Double-stranded RNAi was used to evaluate a number of different genes in ARE-mediated RNA stability in S2 cells. The RNAi treatments were performed as described in the Experimental Procedure. RT-PCR was used to confirm the knockdown of corresponding mRNA. Real-time PCR(More)
Background Cancer is the second leading cause of death worldwide, and oral cancer ranks tenth among all types [1]. Chemotherapy, radiotherapy and surgery are current therapeutic options; however these are not fully efficient. Permanent functional impairment and aesthetic scars are frequent [2]. In this scenario, it is crucial to find therapeutic(More)
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