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The levels of 5-hydroxyindoleacetic acid (5-HIAA) and free and total 5-hydroxytryptophol (5-HTOL) in human and rat brain regions and plasma were determined by a specific capillary column gas chromatographic-mass spectrometric method. The human brains were obtained 2–3 hourspost mortem, and the levels of 5-HIAA were in the range of 0.48–31.3 nmoles/g in the(More)
The serotonin metabolite 5-hydroxytryptophol was determined in cerebrospinal fluid and urine of alcoholics and healthy subjects, by a glass capillary gas chromatographic-mass spectrometric method. The urinary excretion rate (14.6±2.9 pmoles/μmoles creatinine) and urine (109±20 pmoles/ml) and cerebrospinal fluid (4.12±0.21 pmoles/ml) concentrations in(More)
A capillary column gas chromatographic-mass spectrometric method was used to identify and quantitate 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-beta-carboline (6OMTHBC) in rat. The excretion rate in urine was 0.73 +/- 0.20 nmoles per 24 hr and in faeces 0.18 +/- 0.03 nmoles per 24 hr. In urine, about 90% of the 6OMTHBC was in a conjugated form, whereas in faeces(More)
A glass capillary gas chromatographic-mass spectrometric method for the analysis of 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-beta-carboline in urine, plasma and blood platelets was developed. The method involves the use of a deuterated analogue as internal standard, addition of semicarbazide, extraction with methylene chloride and formation of(More)
The occurrence and concentration of the four 5-methoxyindoles: 5-methoxytryptamine, 5-methoxytryptophol, 5-methoxyindole-3-acetic acid and melatonin in the pineal gland of pig, cow, sheep and rat was investigated. The analytical method involved the use of deuterated analogues as internal standards and capillary column gas chromatography — mass spectrometry.(More)
A gas chromatography-mass spectrometry method is described for the identification and quantitation of 6-hydroxy-1,2,3,4-tetrahydro-beta-carboline (6OHTHBC). During the analysis of 6OHTHBC in human platelets, the artifactual formation of 6OHTHBC was observed and shown to be due to the presence of formaldehyde in both organic solvents and in platelet(More)
The two serotonin metabolites 5-hydroxyindoleacetic acid (5HIAA) and 5-hydroxytryptophol (5HTOL) were measured in two regions of rat brain (pons medulla and diencephalon) using a gas chromatographic-mass spectrometric (GC-MS) method. Acute ethanol intoxication effected an elevation of 5-hydroxytryptophol levels, while 1 week of treatment with ethanol(More)