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  • N. V. Sergeev, N S Gloukhova, +4 authors Anatoly I. Miroshnikov
  • Chemistry, Medicine
  • Journal of chromatography. A
  • 2001
  • An analytical scheme for monitoring recombinant human insulin (rhI) production is suggested. The scheme includes high-performance separation micro-techniques (narrow-bore RP-HPLC, HPCE) based onExpand
  • Roman S. Esipov, Vasily N Stepanenko, Larisa A Chupova, Uljana Boyarskikh, Maxim Filipenko, Anatoly I. Miroshnikov
  • Medicine, Biology
  • Protein expression and purification
  • 2008 (First Publication: 1 September 2008)
  • Chemical-enzymatic synthesis of human Epidermal Growth Factor (hEGF) cDNA has been performed, following by cloning into expression vector pTWIN1 (New England Biolabs). The resulting recombinantExpand
  • Roman S. Esipov, Alexandr I Gurevich, Dmitry V Chuvikovsky, Larisa A Chupova, Tatyana I. Muravyova, Anatoly I. Miroshnikov
  • Biology, Medicine
  • Protein expression and purification
  • 2002 (First Publication: 1 February 2002)
  • The Escherichia coli genes encoding purine nucleoside phosphorylase, uridine phosphorylase, and thymidine phosphorylase were cloned into pET plasmids to generate highly effective E. coli BL21(DE3)Expand
  • Andrey Yagodkin, Alex Azhayev, +6 authors Hugh Mackie
  • Medicine, Chemistry
  • Nucleosides, nucleotides & nucleic acids
  • 2007 (First Publication: 15 June 2007)
  • A new method to produce a set of 20 high quality trinucleotide phosphoramidites on a 5–10 g scale each was developed. The procedure starts with condensation reactions of P-components with N-acylExpand