Anatoliy I. Dragan

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PicoGreen is a fluorescent probe that binds dsDNA and forms a highly luminescent complex when compared to the free dye in solution. This unique probe is widely used in DNA quantitation assays but has limited application in biophysical analysis of DNA and DNA-protein systems due to limited knowledge pertaining to its physical properties and characteristics(More)
PicoGreen (PG) is a fluorescent probe for both double-stranded DNA (dsDNA) detection and quantification based on its ability to form a luminescent complex with dsDNA as compared with the free dye in solution. To expand the sensitivity of PG detection, we have studied the spectral properties of PG, both free and in complex with DNA in solution, when the(More)
In this study we have analyzed metal-enhanced fluorescence (MEF) effects from different density silver island films (SiFs) and the effects of sample geometry on the observed enhancement of fluorescence (EF). It is shown that silver islands grow exponentially with SiF deposition time (DT<7min), optical density of SiFs almost linearly depends on DT;(More)
Metal-Enhanced Fluorescence (MEF) effects from different density silver island films (SiFs) and the effects of far-field excitation irradiance on the observed enhancement of fluorescence were studied. It is shown that MEF non-linearly depends on silver nanoparticle (NP) size/density, reaching a maximum value for SiFs made at a deposition time (DT) of ∼5(More)
In this paper, metal-enhanced fluorescence (MEF) from 6-propionyl-2-dimethylaminonaphthalene (Prodan) in different solvents when placed in close proximity to silver island films (SIFs), surface-deposited nanoparticles, is studied. We observe that MEF is wavelength dependent: the enhanced emission of Prodan on SIFs in different solvents changes from 1.5to(More)
Tyrosyl-tRNA synthetase from Mycobacterium tuberculosis (MtTyrRS) is an enzyme that belongs to class I of aminoacyl-tRNA synthetases, which catalyze the attachment of l-tyrosine to its cognate tRNATyr in the preribosomal step of protein synthesis. MtTyrRS is incapable of cross-recognition and aminoacylation of human cytoplasmic tRNATyr, so this enzyme may(More)
Ethidium bromide (EB) is a commonly used probe for fluorescence detection and quantification of nucleic acids, since EB forms a highly luminescent complex with dsDNA. Typical detection sensitivity of EB-based assays to dsDNA in solution is about 0.1 lg. With the aim to increase the sensitivity of EB assays we have employed the Metal-Enhanced Fluorescence(More)
In this study, we have investigated the fluorescence properties of SYBR Green I (SG) dye and its interaction with double-stranded DNA (dsDNA). SG/dsDNA complexes were studied using various spectroscopic techniques, including fluorescence resonance energy transfer and time-resolved fluorescence techniques. It is shown that SG quenching in the free state has(More)
Rapid presymptomatic diagnosis of Bacillus anthracis at early stages of infection plays a crucial role in prompt medical intervention to prevent rapid disease progression and accumulation of lethal levels of toxin. To detect low levels of the anthrax protective antigen (PA) exotoxin in biological fluids, we have developed a metal-enhanced fluorescence(More)
Multilayers of nanoburger structures of silver island films-SiO(2)-silver island films (SIFs-SiO(2)-SIFs) were used as substrates to study the fluorescence of close-proximity fluorophores. Compared to single-layered SIFs, multilayer nanoburgers exhibit several distinctive properties including a significantly enhanced fluorescence intensity, decreased(More)