Anatoliy Anisimov

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Earlier we showed that 4-hours treatment of cells K562 with the GTP-binding protein activator AlF4- (10 mM NaF + 20 microM AlCl3) increased the DNA fragmentation on an average to 5% of the total 3H-thymidine-labeled DNA. The viability of cells under these conditions did not change. It has been suggested that gene toxic action of AlF4- is a result of cell(More)
We studied the ability of inducers and inhibitors of erythroid differentiation of K562 leukemia cells, such as sodium butyrate, dimethyl sulfoxide, and phorbol-12-myristate-13-acetate, respectively, to modulate sensitivity of these cells to non-specific lysis (non-restricted with respect to antigens of the major histocompatibility complex) mediated by(More)
Using trypan blue exclusion test it has been shown that a 18 hour incubation of human erythromyeloleukosis cell line K562 together with AlF(-4) (10 mM NaF + 20 mkM AlCl3) reduced cell proliferation and survival. However, the latter parameter did not change during 4 hours of incubation. Nevertheless, AlF(-4) increased fragmentation of 3H-thymidine-labelled(More)
The literature data on the influence of a large group of cancer cell differentiation inducers on the modulation of their susceptibility to non-MHC-restricted lysis (non-specific cytotoxicity, NCT) by natural killer (NK) cells have been analysed. A possible association between the apoptogenic action of differentiation inducers and their ability to modulate(More)
We studied the effect of certain differentiation inducers-thymidine, sodium butyrate, and dimethyl sulfoxide--on the cells of parental line K562 and the derived sublines resistant to quinoline xenobiotics 2-(4-dimethylaminostyryl)quinoline 1-oxide or 4-nitroquinoline 1-oxide. The cells of the both derived sublines demonstrate cross-resistance to these(More)
The differentiating effect of DMSO on K562 cells was studied against the background of pretreatment of the cells by the modulators of activities of protein kinase C and Ca signaling, phorbol 12-myristate 13-acetate, and ionophore A23187. The 2-hour pretreatment of K562 cells with A23187 (1 microM), rather than phorbol 12-myristate 13-acetate (0.1 microM),(More)
We considered the biological effects of some chemical compounds, that stimulate cell differentiation, on the cells of tumor lines. Induced changes were analyzed in the expression of several transcriptional factors involved in the regulation of cell proliferation and apoptosis. Based on the generalized information about differentiating, apoptogenic, and(More)