Ananda Wijewickrama

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Dengue viral infections are one of the most important mosquito-borne diseases in the world. Presently dengue is endemic in 112 countries in the world. It has been estimated that almost 100 million cases of dengue fever and half a million cases of dengue hemorrhagic fever (DHF) occur worldwide. An increasing proportion of DHF is in children less than 15(More)
The origin of the Sinhalese population of Sri Lanka is debated. We subtyped HLA-A*02 in 101 Sinhalese and observed a preponderance of the rare allele HLA-A*0211 which was similar to reported frequencies in northern India. Taken with low-resolution typing for the remaining A, B, C, DR and DQ alleles, these data suggest a North Indian origin for the Sri(More)
BACKGROUND Liver involvement in acute dengue infection is frequently observed and sometimes leads to acute liver failure, with fatal outcomes. Many factors are thought to contribute to liver dysfunction, including hypoxic injury due to decreased perfusion, direct damage by the virus and immune mediated injury. In this study, we sought to identify the(More)
BACKGROUND In Sri Lanka, varicella zoster virus (VZV) is typically acquired during adulthood with significant associated disease morbidity and mortality. T cells are believed to be important in the control of VZV replication and in the prevention of reactivation. The relationship between viral load, disease severity and cellular immune responses in primary(More)
BACKGROUND Each year dengue virus (DENV) infects 400 million human but causes symptomatic disease in only a subset of patients, suggesting that host genetic factors may play a role. HLA molecules that restrict T-cell responses are one of the most polymorphic host factors in humans. METHODS Here we map HLA DRB1-restricted DENV-specific epitopes in(More)
Background: Dengue viral infections are a major public health problem in many tropical and sub-tropical countries of the world. Early diagnosis and appropriate therapeutic interventions are critical for reducing both morbidity and mortality. The WHO has proposed a revised classification based on warning signs as the validity and usefulness of the WHO 1997(More)
INTRODUCTION Dengue hemorrhagic fever (DHF) is a major cause of morbidity and mortality in tropical regions. Serum free calcium (Ca2+) is known to be important in cardiac and circulatory function. We evaluated association between serum Ca2+ level and severity of dengue. METHODOLOGY A cross-sectional study was carried out at a tertiary care private(More)
INTRODUCTION Genotyping of wild type of varicella zoster virus (VZV) in Sri Lanka would help to distinguish the VZV wild type infection from varicella vaccine associated infections. METHODS PCR-RFLP analysis of VZV ORF 38, 54 and 62 was used for genotyping in VZV from blood or vesicular fluid from 31 patients with chickenpox or herpes zoster. The PstI(More)
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