Anand Balasubramani

Learn More
Distal cis-regulatory elements play essential roles in the T lineage-specific expression of cytokine genes. We have mapped interactions of three trans-acting factors-NF-kappaB, STAT4, and T-bet-with cis elements in the Ifng locus. We find that RelA is critical for optimal Ifng expression and is differentially recruited to multiple elements contingent upon T(More)
Phenotypic plasticity of T helper 17 (Th17) cells suggests instability of chromatin structure of key genes of this lineage. We identified epigenetic modifications across the clustered Il17a and Il17f and the Ifng loci before and after differential IL-12 or TGF-beta cytokine signaling, which induce divergent fates of Th17 cell precursors. We found that Th17(More)
Study of the development of distinct CD4(+) T-cell subsets from naive precursors continues to provide excellent opportunities for dissection of mechanisms that control lineage-specific gene expression or repression. Whereas it had been thought that the induction of transcription networks that control T-lineage commitment were highly stable, reinforced by(More)
Three recent studies, including one in this issue of Molecular Cell, document unexpected physical and functional interactions between two unrelated enzymes: OGT, which transfers O-GlcNAc to serine/threonine residues of numerous cellular proteins, and TET-family dioxygenases, which successively oxidize 5-methylcytosine in DNA.
ASXL1 is the obligate regulatory subunit of a deubiquitinase complex whose catalytic subunit is BAP1. Heterozygous mutations of ASXL1 that result in premature truncations are frequent in myeloid leukemias and Bohring-Opitz syndrome. Here we demonstrate that ASXL1 truncations confer enhanced activity on the ASXL1-BAP1 complex. Stable expression of truncated,(More)
Differentiation-dependent regulation of the Ifng cytokine gene locus in T helper (Th) cells has emerged as an excellent model for functional study of distal elements that control lineage-specific gene expression. We previously identified a cis-regulatory element located 22 kb upstream of the Ifng gene (Conserved Non-coding Sequence -22, or CNS-22) that is a(More)
Viruses compound dietary pathology Reoviruses commonly infect humans and mice asymptomatically. Bouziat et al. found that immune responses to two gutinfecting reoviruses take different paths in mice (see the Perspective by Verdu and Caminero). Both reoviruses invoked protective immune responses, but for one reovirus, when infection happened in the presence(More)
Start with the transition state Zeolites catalyze a wide range of industrial reactions, but identifying the reactions that they catalyze well tends to be done by trial and error. Gallego et al. propose a way to rationalize the process by using structure-directing agents that act as scaffolds for creating the zeolite pores and channels (see the Perspective(More)
ATP boosts protein solubility Adenosine triphosphate (ATP) has well-characterized roles in providing energy for biochemical reactions within cells. Patel et al. find that ATP may also enhance protein solubility, which could help explain why such high concentrations of ATP are maintained in cells (see the Perspective by Rice and Rosen). Protein(More)
Pattern formation in the brain Neurons in the developing brain cooperate to build circuits. Mountoufaris et al. found that ~50 variable protocadherin genes support a combinatorial identity code that allows millions of olfactory neuron axons to sort into ~2000 glomeruli. Sharing olfactory receptors drives axons to one glomerulus, and protocadherin diversity(More)