Ana Smolko

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A novel activity of halohydrin dehalogenases towards spiroepoxides has been found. The enzyme from Arthrobacter sp. (HheA) catalysed highly regioselective azidolysis of spiroepoxides containing 5, 6 and 7-membered cycloalkane rings, while the enzyme from Agrobacterium radiobacter (HheC), besides high regioselectivity, also displayed moderate to high(More)
In this study cellular proteins of in vitro grown horseradish (Armoracia lapathifolia Gilib.) tissues of different morphologies, leaves (L), teratoma (TM) and tumor (TN), were separated by one-dimensional (1-DE) and two-dimensional polyacrylamide gel electrophoresis (2-DE) in order to reveal developmentally specific proteins. Moreover, proteins were(More)
Stress hormones, particularly jasmonic acid, influenced root growth, auxin levels, and transcription of auxin amidohydrolase BrIAR3 in Brassica rapa seedlings, while auxin conjugate synthetases BrGH3.1 and BrGH3.9 were down-regulated by all treatments. The influence of stress hormones: jasmonic acid (JA), salicylic acid (SA), and abscisic acid (ABA) on(More)
Brassica rapa auxin amidohydrolase (BrILL2) participates in the homeostasis of the plant hormones auxins by hydrolyzing the amino acid conjugates of auxins, thereby releasing the free active form of hormones. Herein, the potential role of the two conserved Cys residues of BrILL2 (at sequence positions 139 and 320) has been investigated by using(More)
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