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Shoot-tips and somatic embryos are the explants of choice for the in vitro long-term storage of ex situ plant genetic resources in liquid nitrogen. Cryopreservation of organized structures has significantly progressed, especially for species of tropical origin, with the development of several vitrification-based procedures such as encapsulation-dehydration,(More)
This study was carried to identify the causes of pancytopenia and to find out the bone marrow morphology in cases of pancytopenia. It was a cross sectional study conducted over a period of two years in the Department of Pathology, Tribhuvan University Teaching Hospital. Bone marrow aspiration smears of patients fulfilling the criteria of pancytopenia were(More)
BACKGROUND CIP maintains the largest in vitro clonal potato collection in the world, comprising 4,013 landraces and 3,353 improved accessions. The in vitro technology is more efficient and secure than conservation in the field, allowing in vitro plantlets to be stored for approximately 2 years without sub-culture. This method however is not ideal for the(More)
BACKGROUND Cryobanks are a secure, efficient and low cost method for the long-term conservation of plant genetic resources for theoretically centuries or millennia with minimal maintenance. OBJECTIVE The present manuscript describes CIP's modified protocol for potato cryopreservation, its large-scale application, and the establishment of quality and(More)
The Latin America and Caribbean (LAC) region harbors major centers of origin and diversity for a number of organisms that support current world food production and industry. Agriculture and biodiversity are closely linked to biotechnology; the sustainability of protected biodiversity areas depends to a large extent on what transpires in intervened adjacent(More)
The effect of cold and sucrose pretreatment for increasing tolerance to cryopreservation was evaluated with eight diverse genotypes, six cultivars belonging to the cultivated species, Solanum tuberosum spp., S. tuberosum subsp. andigena, S. x juzepczukii and S. x ajanhuiri, and two genotypes from the wild species, S. commersonii. In vitro plantlets were(More)
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